Table S1. Bacterial strains and plasmids used in this work.

Table S2. Oligonucleotides used in this work.

Fig. S1. Phenotypic confirmation of the biofilm-persistent mutants. Overnight cultures were diluted 50-fold in LB and inoculated in octuplicate in 96-well microtitre dishes. The dishes were incubated for 24 h at 25°C with moderate shaking (150 r.p.m.) and processed for planctonic (open bars) and biofilm (closed bars) biomass analysis. Bars represent the average and standard deviations of at least three biological replicates.

Fig. S2. Location of the miniTn5-Km insertions in the biofilm-persistent mutants. Cartoons showing sections of the P. putida KT2440 chromosome around the transposon insertions are shown for PP0164 (A), PP0914 (B), PP3540 (C), and PP4693 (D). Genes bearing the insertions are shown as open pointed boxes. Other genes are shown as shaded pointed boxes. Absolute coordinates of the P. putida KT2440 genome are shown below the genes. Transposon insertions are denoted by inverted triangles. Each panel drawn to scale.

Experimental procedures.

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