Detailed experimental procedures

Fig. S1. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) tree showing phylogenetic relationships of KS-AT domains from different types of PKS and FAS enzymes.

Fig. S2. Alignment of KS domains from type I PKSs and FASs.

Fig. S3. Alignment of AT domains from type I PKSs and FASs.

Fig. S4. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel showing the successful expression of the N-terminal His8-tag fusion proteins apo-SwfAACP and holo-SwfAACP before and after purification using nickel-nitriloacetic acid (Ni-NTA) columns.

Fig. S5. Analysis of the fine structure of the sixfold charged ion peak of holo-SwfAACP.

Fig. S6. PCR detection of swf genes in seven different ‘high microbial abundance’ marine sponges.

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