Polyketide genes in the marine sponge Plakortis simplex: a new group of mono-modular type I polyketide synthases from sponge symbionts
Version of Record online: 25 JUL 2013
© 2013 The Authors. Environmental Microbiology Reports published by John Wiley & Sons Ltd and Society for Applied Microbiology.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Environmental Microbiology Reports
Volume 5, Issue 6, pages 809–818, December 2013
How to Cite
Della Sala, G., Hochmuth, T., Costantino, V., Teta, R., Gerwick, W., Gerwick, L., Piel, J. and Mangoni, A. (2013), Polyketide genes in the marine sponge Plakortis simplex: a new group of mono-modular type I polyketide synthases from sponge symbionts. Environmental Microbiology Reports, 5: 809–818. doi: 10.1111/1758-2229.12081
- Issue online: 14 NOV 2013
- Version of Record online: 25 JUL 2013
- Accepted manuscript online: 26 JUN 2013 12:32PM EST
- Manuscript Accepted: 22 JUN 2013
- Manuscript Received: 16 FEB 2013
- European Commission. Grant Numbers: 229893, 311848
- Ministero dell'Istruzione, dell'Università e della Ricerca. Grant Number: PRIN2009
- National Institutes of Health. Grant Number: CA108874
- Deutsche Forschungsgemeinschaft. Grant Number: PI 430/8-1
Detailed experimental procedures
Fig. S1. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) tree showing phylogenetic relationships of KS-AT domains from different types of PKS and FAS enzymes.
Fig. S2. Alignment of KS domains from type I PKSs and FASs.
Fig. S3. Alignment of AT domains from type I PKSs and FASs.
Fig. S4. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel showing the successful expression of the N-terminal His8-tag fusion proteins apo-SwfAACP and holo-SwfAACP before and after purification using nickel-nitriloacetic acid (Ni-NTA) columns.
Fig. S5. Analysis of the fine structure of the sixfold charged ion peak of holo-SwfAACP.
Fig. S6. PCR detection of swf genes in seven different ‘high microbial abundance’ marine sponges.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.