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Fig. S1. Schematic representation of the AMT cruise track and the studied stations (indicated by numbers).

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Fig. S2. Confocal microscope images of Chrysophyceae/SAR11 cells (A) and Pelagophyceae/Prochlorococcus cells (B). Yellow-green colour shows the positive signal of the HRP-labelled prokaryotic probe, and the red colour shows the positive signal of the eukaryotic probe. The scale bar is 5 μm and applies to all figures.

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Fig. S3. Fluorescence microscope image of a Prochlorococcus cell attached to a Prymnesiophyceae cell from the northern subtropical gyre. (A) The orange and green colour shows the positive signal of the Prymnesiophyceae and Prochlorococcus probes respectively. (B) DAPI staining of the same cells. The scale bar is 5 μm for each image.

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Fig. S4. Cell abundance of low nucleic acid bacteria (LNA, i.e. SAR11) and Prochlorococcus (Pro) in the Northern subtropical gyre (NG), equatorial region (EQ) and the Southern subtropical gyre (SG).

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