SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
emi412088-sup-0001-fig_s1.pdf31K

Fig. S1. Phylogenetic context for the 16S rRNA gene of the Euryarchaeota DHVE and MEG targeted by specific primers in this study (SILVA ribosomal database 111 RefNR, July 2012). The clades are highlighted and equivalent clade nomenclature has been added following Durbin and Teske (2011; 2012). Scale bar, 10% estimated divergence.

emi412088-sup-0002-fig_s2.ai1679K

Fig. S2. Specificity level (number of bands observed in an agarose gel) observed for the newly designed primers at different PCR annealing temperatures. Averaged values and error range for PCR products obtained from lakes Bergús, Llebreta and Muntanyó d'Arreu. The primers set MEG 93f-392r (expected PCR product size ∼ 296 bp) and DSEG 510f-725r (∼ 216 bp) showed the best performance and specificity.

emi412088-sup-0003-fig_s3.pdf26K

Fig. S3. Maximum-likelihood phylogenetic tree for the 16S rRNA gene of the Euryarchaeota clade MEG (Miscellaneous Euryarchaeota Group comprising the clades pMC2A384 and VALII/Eury4; Durbin and Teske, 2012) targeted by the specific primers set designed in this study. Scale bar, 10% estimated divergence.

emi412088-sup-0004-fig_s4.pdf36K

Fig. S4. Maximum-likelihood phylogenetic tree for the 16S rRNA gene of the Euryarchaeota clade DSEG (Deep-Sea Euryarchaeotal Groups, targeting the cluster named VALIII comprising the DHVE-3/DSEG, BC07-2A-27/DSEG-3 and DSEG-2 groups; Durbin and Teske, 2011; 2012) targeted by the specific primer set designed in this study. Scale bar, 10% estimated divergence.

emi412088-sup-0005-table_s1.pdf138K

Table S1. NCBI accession numbers of the 16S rRNA gene sequences used to design the new and specific primers for Euryarchaeotal clades MEG and DSEG in combination with the Pyrenean 16S rRNA gene dataset.

emi412088-sup-0006-table_s2.pdf153K

Table S2. Specific primers set designed in this study for qPCR analysis of the euryarchaeotal clades MEG and DSEG. The primer sets MEG 93f-392r, and DSEG 510f-725r showed the best performance and specificity, respectively (see Fig S2).

emi412088-sup-0007-table_s3.pdf75K

Table S3. Environmental parameters of the different lakes analysed in this study and specific 16S rRNA gene abundances for the Euryarchaeota DSEG and MEG, and Thaumarchaeota SAGMGC populations. Standard deviations < 10% applies for all the qPCR data. N/A not available; N/D not detected.

emi412088-sup-0008-table_s4.pdf105K

Table S4. Environmental parameters for the different dates and depths analysed in Lake Redon and specific 16S rRNA gene abundances for the Euryarchaeota DSEG and MEG, and Thaumarchaeota SAGMGC populations. Standard deviations < 10% applies for all the qPCR data. N/A not available; N/D not detected.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.