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Successful enrichment of the ubiquitous freshwater acI Actinobacteria

Authors

  • Sarahi L. Garcia,

    1. Jena School for Microbial Communication (JSMC) and Microbial Ecology Group at Friedrich Schiller University, Jena, Germany
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  • Katherine D. McMahon,

    1. Department of Civil and Environmental Engineering, Department of Bacteriology, University of Wisconsin, Madison, WI, USA
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  • Hans-Peter Grossart,

    1. Leibniz-Institute for Freshwater Ecology and Inland Fisheries, Department 3: Limnology of Stratified Lakes, Stechlin, Germany
    2. Institute for Biochemistry and Biology, Potsdam University, Potsdam, Germany
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  • Falk Warnecke

    Corresponding author
    1. Jena School for Microbial Communication (JSMC) and Microbial Ecology Group at Friedrich Schiller University, Jena, Germany
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Summary

Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for > 50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater Actinobacteria, diluted freshwater samples from Lake Grosse Fuchskuhle, Germany, were incubated in 96-well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acI Actinobacteria of the enrichment belonged to the acI-B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI-B2 was confirmed.

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