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emi412106-sup-0001-si.tif14692K

Fig. S1. Phylogenetic tree of rbcL protein sequences annotated in microalgal genomes. Tree is arbitrarily rooted. Accession numbers are indicated between parentheses. The phylogenetic tree was inferred by maximum likelihood with the RtRev + I + G + F model of protein evolution. Branch support was calculated with the approximate likelihood ratio test (aLRT) and indicated on the branches (values < 50% are not shown). The scale bar indicates evolutionary distance of 0.1 substitutions per site.

emi412106-sup-0002-si.tif2684K

Fig. S2. Alignment of the CS DNA fragment comprising the designed primers in the sequences from diatom cultures. Black bars indicate DNA sequence area comprising the Cycloart_F and R primers, and yellow bars indicate DNA sequence area comprising the CycloF_TPF and CycloR_TPF primers (see Table S2 for details).

emi412106-sup-0003-si.tif3635K

Fig. S3. Alignment of the CS protein fragment comprising the designed primers in the sequences from diatom cultures. Black bars indicate protein sequence area comprising the Cycloart_F and R primers, and yellow bars indicate protein sequence area comprising the CycloF_TPF and CycloR_TPF primers (see Table S2 for details). Stars indicate amino acid positions in which amino acid changes have been observed between the CS sequences of S. costatum and S. subsalsum. The amino acid positions in this alignment are numbered according to the CS sequence of T. pseudonana (accession number XP_002287432.1).

emi412106-sup-0004-si.tif2014K

Fig. S4. Distribution of introns/exons in the CS gene fragment comprised by the primers designed in this study. Exon areas are indicated with blue boxes and introns as lines. aa, amino acids; Bp, base pairs.

emi412106-sup-0005-si.doc101K

Table S1. Annotated CS orthologs in microalgal genomes.

Table S2. List of primers used for the detection of CS gene coding sequences.

Table S3. Diatom cultures investigated in this study.

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