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emi412107-sup-0001-si.doc38K

Appendix S1 Viability patterns of myxobacterial eco-types.

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Fig. S1. The proportion of myxobacterial suborders and unclassified myxobacterial sequences in different soil samples from SRA database.

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Fig. S2. The relationship between relative abundance of myxobacteria and the environmental parameters including texture (A), c min rate (B) and the mean annual precipitation (C) of different soil samples. Spearman's rank correlation (r) and P value were shown in Fig. Solid circles represent the soils from MG-RAST database and unfilled triangle represents the SDU soil sample.

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Fig. S3. Phylogenetic analysis of metabolically active myxobacterial sequences (16S crDNA). The phylogenetic tree was calculated using interior branch test of phylogeny program by neighbour joining method in MEGA 4. The bar indicates the nucleotides changes per 1 bp. The bootstrapping supports for the trees were calculated from a sample of 1000 replicates. The reference sequences were extracted from the GenBank databases. D. desulphuricans (GenBank Accession no. M34113) was used to root the tree. The outermost cycle was added following construction.

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Table S1. Summary of pyrosequencing data of SDU soil sample.

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Table S2. Bacterial community structure in the universal libraries of SDUUV34 and SDUUV678 classified on RDP website.

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Table S3. The taxonomic composition of myxobacteria in the universal libraries of SDUUV34 and SDUUV678

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Table S4. Information of pyrosequencing datasets and site locations for investigated soils.

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Table S5. The taxonomic composition of myxobacteria in the Cystobacterineae-enriched SDUCV34 library.

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Table S6. The taxonomic composition of myxobacteria in the Sorangiineae-enriched SDUSV678 library.

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Table S7. Strains and their 16S rDNA GenBank accession numbers used in the phylogenetic tree.

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