SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
emi412109-sup-0001-si.pdf1408K

Fig. S1. Map of Llaima volcano showing the years of recent lava eruptions and the location of sampling sites (modified from Naranjo and Moreno, 2005). Exact positions were obtained by global positioning system data and are detailed in Table S1.

Fig. S2. Nitrate concentration during incubation of three different sampling sites. Shaken-slurry method of soils incubated with (NH4)2SO4 for 28 days were analysed. Soils were incubated in absence and presence of 100 Pa C2H2. Bars represent standard deviations of triplicate incubations. Different letters above the bars indicate statistically significant differences between the treatments (one-way analysis of variance with Tukey's post hoc test at P ≤ 0.05).

Fig. S3. Abundance of (A) archaeal and (B) bacterial amoA genes of three different sampling sites from volcanic soils studied in the present study. Shaken-slurry method of soils incubated with (NH4)2SO4 for 28 days were analysed. Soils were incubated in absence and presence of 100 Pa C2H2. Bars represent standard deviations of triplicate samples.

Fig. S4. Constrained correspondence analysis calculated from archaeal amoA T-RFLP data (NlaIII) from fresh volcanic soils (n = 9). T-RFs with normalized peak areas of < 2% were excluded from the analysis. Numbers next to the crosses describe the size of T-RFs.

Table S1. Physico-chemical characteristics of the volcanic soils used in the present study.

Table S2. Primer sets and PCR conditions used in this study.

Table S3. Operational taxonomic unit (OTU) and Shannon index of bacterial and archaeal amoA sequences. OTUs were defined at a sequence identity level of 93% for bacterial amoA and 88% and 87% for archaeal amoA at 0 and 28 days incubation respectively.

Table S4. Number of archaeal (AOA) and bacterial (AOB) amoA gene clones retrieved from different volcanic soils and length of terminal restriction fragments (T-RFs) with different restriction enzymes.

Appendix S1. Experimental procedures.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.