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emi412121-sup-0001-si.tif2279KFig. S1. Monthly isolation of O1 and O139 V. cholerae strains from Zhujiang estuary during the 1-year period. Positive rate means the monthly isolation rate of both O1 and O139 strains from the water samples.
emi412121-sup-0002-si.tif1369KFig. S2. Alignment of rstR nucleotide sequences from representative strains and Zhujiang V. cholerae isolates. ig-1 and rstA sequences (partial) are indicated with black lines at the bottom of all compared sequences. The start and stop codons of each rstR are marked with ‘###’ and ‘***’, respectively, above each sequence. The start codons of rstA are marked with a black arrow at the top of the compared sequences. GenBank accession numbers of each rstR type and strain are as follows: N16961, AE004224; 569B, AF055890; 506-94, AF452586; AS207, AF110029; SCE223, AF133307; SEC264, AF133308; VCE232, DQ288668; and VC06–18, HM590227.
emi412121-sup-0003-si.tif1953KFig. S3. Alignment of pIIICTX amino acid sequences from Zhujiang V. cholerae isolates. Strains N16961 (O1 El Tor), VCE22 (O36) and JS9803 (O139) are also included. Red lines indicate signal and low-complexity regions (LCRs); gray lines indicate D1, D2 and D3 domains.
emi412121-sup-0004-si.tif5963KFig. S4. Alignment of TcpA amino acid sequences from Zhujiang V. cholerae isolates. Strains N16961 (O1 El Tor), VCE22 (O36) and JS9803 (O139) are also included. The red line indicates the structural domain, and the blue line indicates the interaction domain.
emi412121-sup-0005-si.doc205K

Table S1. Characteristics of V. cholerae O1 and O139 strains isolated in the estuary of Zhujiang River.

Table S2. PCR primers used in this study.

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