These authors contributed equally to this work.
High prevalence and diversity of pre-CTXΦ alleles in the environmental Vibrio cholerae O1 and O139 strains in the Zhujiang River estuary
Article first published online: 5 DEC 2013
© 2013 Society for Applied Microbiology and John Wiley & Sons Ltd
Environmental Microbiology Reports
Volume 6, Issue 3, pages 251–258, June 2014
How to Cite
Wang, D., Wang, X., Li, B., Deng, X., Tan, H., Diao, B., Chen, J., Ke, B., Zhong, H., Zhou, H., Ke, C. and Kan, B. (2014), High prevalence and diversity of pre-CTXΦ alleles in the environmental Vibrio cholerae O1 and O139 strains in the Zhujiang River estuary. Environmental Microbiology Reports, 6: 251–258. doi: 10.1111/1758-2229.12121
- Issue published online: 5 MAY 2014
- Article first published online: 5 DEC 2013
- Accepted manuscript online: 1 NOV 2013 05:40AM EST
- Manuscript Accepted: 27 OCT 2013
- Manuscript Received: 13 AUG 2012
- National Natural Science Foundation of China. Grant Number: 30872260
- Key Project of NSFC. Grant Number: 30830008
- National Basic Research Priorities Program. Grant Number: 2009CB522604
- Guangzhou Medical and Health Science and Technology Projects. Grant Number: 2008-ZDa-06
|emi412121-sup-0001-si.tif||2279K||Fig. S1. Monthly isolation of O1 and O139 V. cholerae strains from Zhujiang estuary during the 1-year period. Positive rate means the monthly isolation rate of both O1 and O139 strains from the water samples.|
|emi412121-sup-0002-si.tif||1369K||Fig. S2. Alignment of rstR nucleotide sequences from representative strains and Zhujiang V. cholerae isolates. ig-1 and rstA sequences (partial) are indicated with black lines at the bottom of all compared sequences. The start and stop codons of each rstR are marked with ‘###’ and ‘***’, respectively, above each sequence. The start codons of rstA are marked with a black arrow at the top of the compared sequences. GenBank accession numbers of each rstR type and strain are as follows: N16961, AE004224; 569B, AF055890; 506-94, AF452586; AS207, AF110029; SCE223, AF133307; SEC264, AF133308; VCE232, DQ288668; and VC06–18, HM590227.|
|emi412121-sup-0003-si.tif||1953K||Fig. S3. Alignment of pIIICTX amino acid sequences from Zhujiang V. cholerae isolates. Strains N16961 (O1 El Tor), VCE22 (O36) and JS9803 (O139) are also included. Red lines indicate signal and low-complexity regions (LCRs); gray lines indicate D1, D2 and D3 domains.|
|emi412121-sup-0004-si.tif||5963K||Fig. S4. Alignment of TcpA amino acid sequences from Zhujiang V. cholerae isolates. Strains N16961 (O1 El Tor), VCE22 (O36) and JS9803 (O139) are also included. The red line indicates the structural domain, and the blue line indicates the interaction domain.|
Table S1. Characteristics of V. cholerae O1 and O139 strains isolated in the estuary of Zhujiang River.
Table S2. PCR primers used in this study.
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