emi412132-sup-0001-si.docx2580KFig. S1. Phylogenetic tree of attine ant associated Pseudonocardia and their free-living counterparts. The tree was inferred using the maximum likelihood algorithm under the Tamura-3 parameters substitution model. Values on branches are bootstrap support calculated with 1000 pseudoreplicates. Sequence in bold refers to Pseudonocardia sp. TD007. Sequences in clades II and III along with sequences of free-living Pseudonocardia (see Cafaro et al., 2011) were retrieved from the NCBI-GenBank. Sequences highlighted in gray comprise Pseudonocardia strains associated with attine ants followed by the ant species name and nest ID (in parentheses). Pseudonocardia halofobica was used as outgroup following Cafaro and colleagues (2011).
emi412132-sup-0001-si.docx2580KFig. S2. Bioassays between filamentous fungi and Actinobacteria. Two ant-associated Pseudonocardia (TD003 and TD031) and two Streptomyces strains isolated from soil (SC048 and SC049) were tested against the four Escovopsis strains and three soil-borne filamentous fungi (Trichoderma sp. SC076, Mucor sp. SC005 and Fusarium solani SC006). Only the results for Escovopsis RS055 are shown because both Streptomyces totally inhibited all Escovopsis strains and the results of EscovopsisPseudonocardia interactions are already denoted in Fig. 2.
emi412132-sup-0001-si.docx2580KTable S1. Actinobacteria strains used in this study.
emi412132-sup-0001-si.docx2580KTable S2. Escovopsis strains used in this study.
emi412132-sup-0001-si.docx2580KTable S3. Relative mycelium growth and standard errors of the four Escovopsis strains in bioassays challenges against Actinobacteria associated with Trachymyrmex ants. Figures were obtained by dividing the Escovopsis final growth area in the presence of Actinobacteria (challenges) by the growth areas in the absence of Actinobacteria (control).

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