Control of sulfidogenesis through bio-oxidation of H2S coupled to (per)chlorate reduction
Version of Record online: 4 APR 2014
© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd
Environmental Microbiology Reports
Volume 6, Issue 6, pages 558–564, December 2014
How to Cite
Gregoire, P., Engelbrektson, A., Hubbard, C. G., Metlagel, Z., Csencsits, R., Auer, M., Conrad, M. E., Thieme, J., Northrup, P. and Coates, J. D. (2014), Control of sulfidogenesis through bio-oxidation of H2S coupled to (per)chlorate reduction. Environmental Microbiology Reports, 6: 558–564. doi: 10.1111/1758-2229.12156
- Issue online: 28 NOV 2014
- Version of Record online: 4 APR 2014
- Accepted manuscript online: 5 MAR 2014 05:13AM EST
- Manuscript Accepted: 1 MAR 2014
- Manuscript Received: 11 DEC 2013
- Energy Biosciences Institute
- Office of Science
- Office of Basic Energy Sciences
- U.S. Department of Energy. Grant Number: DE-AC02-05CH11231
Fig. S1. (A) Growth coupled to lactate oxidation and chlorate reduction by a reinoculated culture of A. suillum in unfiltered spent broth after the initial sulfide oxidation was complete. The results depicted are the average of triplicate cultures. (B) Growth coupled to lactate oxidation and chlorate reduction by a reinoculated culture of A. suillum in 0.22 μm filtered spent broth after the initial sulfide oxidation was complete. The results depicted are the average of triplicate cultures.
Fig. S2. Growth coupled to lactate oxidation and chlorate reduction by the static H2S-oxidizing culture of A. suillum when it is used to inoculate fresh BM amended with lactate (10 mM) and chlorate (10 mM).
Fig. S3. X-ray absorption near edge structure (XANES) spectroscopy of washed culture retentate of A. suillum initially and after 24 h incubation in the presence of H2S and chlorate.
Fig. S4. EDX spectra confirming three sulfur inclusions in A. sullium on the carbon film support. Chemical analyses of the whole mount bacterial cell TEM samples were carried out in a JEOL 2100-F 200 kV Field-Emission Analytical Transmission Electron Microscope (TEM) equipped with Oxford INCA Energy Dispersive Spectroscopy (EDX) X-ray detection system at the Molecular Foundry at LBL. EDX spectra were acquired for 60 live seconds with a 0.4 μm probe at 200 kV.
Appendix S1. Supporting information text.
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