High sensitivity of 454 pyrosequencing for detection of rare species in aquatic communities
Article first published online: 22 FEB 2013
© 2013 The Authors. Methods in Ecology and Evolution © 2013 British Ecological Society
Methods in Ecology and Evolution
Volume 4, Issue 6, pages 558–565, June 2013
How to Cite
Zhan, A., Hulák, M., Sylvester, F., Huang, X., Adebayo, A. A., Abbott, C. L., Adamowicz, S. J., Heath, D. D., Cristescu, M. E., MacIsaac, H. J. (2013), High sensitivity of 454 pyrosequencing for detection of rare species in aquatic communities. Methods in Ecology and Evolution, 4: 558–565. doi: 10.1111/2041-210X.12037
- Issue published online: 7 JUN 2013
- Article first published online: 22 FEB 2013
- Manuscript Accepted: 22 JAN 2013
- Manuscript Received: 20 OCT 2012
- Natural Sciences and Engineering Research Council of Canada
- Canadian Aquatic Invasive Species Network
- Ontario Ministry of Research and Innovation Early Researcher Award
- NSERC Discovery Accelerator Supplement
- Chinese Academy of Sciences
- alien invasive species;
- endangered species;
- next-generation sequencing (NGS);
- non-indigenous species;
- nSSU rDNA ;
- universal primers
- Concerns regarding the rapid loss of endemic biodiversity, and introduction and spread of non-indigenous species, have focused attention on the need and ability to detect species present in communities at low abundance. However, detection of rare species poses immense technical challenges, especially for morphologically cryptic species, microscopic taxa and those beneath the water surface in aquatic ecosystems.
- Next-generation sequencing technology provides a robust tool to assess biodiversity, especially for detection of rare species. Here, we assess the sensitivity of 454 pyrosequencing for detection of rare species using known indicator species spiked into existing complex plankton samples. In addition, we develop universal small subunit ribosomal DNA primers for amplification of a wide range of taxa for detailed description of biodiversity in complex communities.
- A universality test of newly designed primers for the hypervariable V4 region of the nuclear small subunit ribosomal DNA (V4-nSSU) using a plankton sample collected from Hamilton Harbor showed that 454 pyrosequencing based on this universal primer pair can recover a wide range of taxa, including animals, plants (algae), fungi, blue-green algae and protists.
- A sensitivity test showed that 454 pyrosequencing based on newly designed universal V4-nSSU primers was extremely sensitive for detection of very rare species. Pyrosequencing was able to recover spiked indicator species with biomass percentage as low as approximately 2·3 × 10−5% when 24 artificially assembled samples were tagged and sequenced in one PicoTiter plate (i.e. sequencing depth of an equivalent of 1/24 PicoTiter plate). In addition, spiked rare species were sometimes recovered as singletons (i.e. Operational Taxonomic Units represented by a single sequence), suggesting that at least some singletons are informative for recovering unique lineages in ‘rare biospheres’.
- The method established here allows biologists to better investigate the composition of aquatic communities, especially for detection of rare taxa. Despite a small-scale pyrosequencing effort, we demonstrate the extreme sensitivity of pyrosequencing using rare species spiked into plankton samples. We propose that the method is a powerful tool for detection of rare native and/or alien species.