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mee312073-sup-0001-TableS1.zipapplication/ZIP13KTable S1. Evaluation of the extraction efficiency of ITSx in nine major groups of eukaryotes. All input sequences covered the full length of the ITS region and featured at least 20 bp of the SSU and LSU genes. Each gene region occupies four columns in the table. Column one indicates the percentage of cases where the gene region was detected at all. Column two indicates the percentage of cases where the gene region was detected and delimited exactly to the correct base-pair. Column three indicates the percentage of cases where the gene region was detected but where the delimitation was one base-pair off. Column four indicates the percentage of cases where the gene region was detected but where the delimitation was two to five base-pairs off.
mee312073-sup-0002-ItemS1-S4.zipapplication/ZIP6007K

Item S1. The software package together with its documentation and a test data set.

Item S2. The test data sets used to evaluate ITSx, plus the output files from the analyses.

Item S3. The output from the evaluation of the pyrosequencing data sets.

Item S4. The output from the one million sequence runs of random DNA sequence data for evaluation of the susceptibility of ITSx to false-positive extractions.

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