Protective effects of Lactobacillus rhamnosus GG against human rotavirus-induced diarrhoea in a neonatal mouse model

Authors

  • Zhen Zhang,

    1. Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • Yun Xiang,

    1. Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • Na Li,

    1. Division of Life Sciences, Graduate School at Shenzhen, Center for Biomedicine and Biotechnology, Tsinghua University, Shenzhen, China
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  • Baoxiang Wang,

    1. Department of Gastroenterology, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • Hongwu Ai,

    1. Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • Xiaomei Wang,

    1. Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • Laiqiang Huang,

    1. Division of Life Sciences, Graduate School at Shenzhen, Center for Biomedicine and Biotechnology, Tsinghua University, Shenzhen, China
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  • Yi Zheng

    Corresponding author
    1. Division of Life Sciences, Graduate School at Shenzhen, Center for Biomedicine and Biotechnology, Tsinghua University, Shenzhen, China
    • Department of Laboratory Medicine, Wuhan Medical and Health Center for Women and Children, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
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  • The manuscript deals with an important topic in mucosal immunology - the mechanism of activity by which probiotics protect against rotavirus (RV)-induced diarrhea. The data shows that in an animal model, administration of Lactobacillus rhamnosus GG (LGG) is associated with higher levels of soluble IgA, and modulation of production of cytokines IFN-[gamma] and TNF-α is observed. These findings may contribute to improve prevention and treatment of RV-induced diarrhea in humans.

Correspondence

Yi Zheng, Center for Biomedicine and Biotechnology, Division of Life Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.

Tel.: +86 755 26036068

fax: +86 755 26036052

e-mail: zhengy@sz.tsinghua.edu.cn

Abstract

Group A human rotaviruses (RV) are a leading cause of severe dehydration and gastroenteritis in infants and young children. A large body of evidence suggests that Lactobacillus rhamnosus GG (LGG) has an effect on the incidence and severity of acute RV-induced diarrhoea; however, the timing and dosage of LGG treatment remains controversial. In the present study, a neonatal mouse model with human RV-induced diarrhoea was set up and the pathophysiological characteristics of the animals were examined. Our results indicated that RV-infected mice developed diarrhoea, accompanied by increased secretion of intestinal mucosa sIgA and serum interferon (IFN)-γ, tumour necrosis factor (TNF)-α, as well as decreased serum IgA. In addition, epithelium vacuolation was noticed in the jejunum microvillus of RV-infected mice. After intragastric administration of low (2 × 105 CFU), middle (2 × 107 CFU) or high (2 × 109 CFU) levels of LGG for four consecutive days before or after RV infection respectively, the RV-infected mice showed a shortened duration of diarrhoea and decreased epithelium vacuolation in the jejunum. Administration of a high dose of LGG before the RV infection was found to have better protective effects against RV infection than other regimens. This study demonstrates that the protective effects of LGG against RV-induced diarrhoea are highly correlated with the timing and dosage of LGG administration in neonatal mice.

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