In the context of spinal tuberculosis a study of the inflammatory responses of human multinucleated osteoclasts infected with virulent Mtb is of interest. Intracellular Mtb infection resulted in the rapid growth of Mtb and production of proinflammatory cytokines. In contrast, highly-fused multinucleated osteoclasts incapacitated the production of these cytokines, Mtb escaped from the endosome/phagosome, and led to a different pattern of osteoclast activation with the production of a set of chemokines. These findings indicate that intracellular Mtb infection in multinuclear osteoclasts reprograms osteoclast development via the dysregulation of cytokines and chemokines.
Mycobacterium tuberculosis escapes from the phagosomes of infected human osteoclasts reprograms osteoclast development via dysregulation of cytokines and chemokines
Version of Record online: 10 SEP 2013
© 2013 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved
Pathogens and Disease
Volume 70, Issue 1, pages 28–39, February 2014
How to Cite
Hoshino, A., Hanada, S., Yamada, H., Mii, S., Takahashi, M., Mitarai, S., Yamamoto, K. and Manome, Y. (2014), Mycobacterium tuberculosis escapes from the phagosomes of infected human osteoclasts reprograms osteoclast development via dysregulation of cytokines and chemokines. Pathogens and Disease, 70: 28–39. doi: 10.1111/2049-632X.12082
- Issue online: 12 FEB 2014
- Version of Record online: 10 SEP 2013
- Accepted manuscript online: 8 AUG 2013 08:37AM EST
- Manuscript Accepted: 30 JUL 2013
- Manuscript Revised: 3 JUL 2013
- Manuscript Received: 15 FEB 2013
- KAKENHI. Grant Numbers: 22790359, #22790359
- Japan Society for the Promotion of Science for Young Scientists
Fig. S1. The expression levels of chemokine ligands by Mtb–infected monocytes, pre-osteoclasts, and multinuclear osteoclasts.
Fig. S2. The expression levels of chemokine receptors by monocytes, pre-osteoclasts, and multinuclear osteoclasts after Mtb stimulation.
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