In this paper further evidence of the important role of the chlamydial plasmid in virulence is provided. In both the respiratory and urogenital tract sites, the plasmid-containing version of Chlamydia trachomatis was more virulent than the non-plasmid strain, when analysed in the mouse model. While this has previously been shown with the mouse strain of Chlamydia, this is the first report showing similar virulence characteristics of the human C. trachomatis strains.
Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model
Article first published online: 10 SEP 2013
© 2013 The Authors. Pathogens and Disease published by John Wiley & Sons Ltd on behalf of the Federation of European Microbiological Societies.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Pathogens and Disease
Volume 70, Issue 1, pages 61–69, February 2014
How to Cite
Sigar, I. M., Schripsema, J. H., Wang, Y., Clarke, I. N., Cutcliffe, L. T., Seth-Smith, H. M.B., Thomson, N. R., Bjartling, C., Unemo, M., Persson, K. and Ramsey, K. H. (2014), Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model. Pathogens and Disease, 70: 61–69. doi: 10.1111/2049-632X.12086
The copyright line for this article was changed on 12 August after original online publication.
- Issue published online: 12 FEB 2014
- Article first published online: 10 SEP 2013
- Accepted manuscript online: 23 AUG 2013 09:02AM EST
- Manuscript Revised: 19 AUG 2013
- Manuscript Accepted: 19 AUG 2013
- Manuscript Received: 19 JUL 2013
- Midwestern University intramural funds and by Wellcome Trust. Grant Numbers: 091296, 098051
- Chlamydia ;
We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C. trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C. trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.