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aab12039-sup-0001-FigureS1.pdfPDF document88KFig. S1. Technical and biological quality assessment of macroarray experiment. (A,B) Correlation between technical replicates. Scater plots comparing normalized signal intensities of the technical replicates for A. thaliana (A) and B. oleracea (B) control sample pairs. Pearson correlation coeficient are presented on the graph; (C) Pearson correlation between the biological replicates. Mean Pearson correlation (R) was calculated for normalized signal intensities of all three pairs of biological replicates; (D) Confirmation rate of the cDNA nylon array ratios with qPCR. Scatter plot of the log2 of the relative change for ABI1 and ERF1 genes in A. thaliana and B. oleracea as determined by qPCR and the cDNA nylon array analysis. The RAB18 gene expression was validated to confirm quality of biological samples used in the macroarray experiment. In general, the qPCR analysis confirms the reliability of the nylon array results. Relative change was calculated as fold change ratio to the non-treated control to indicate genes up- or downregulated, respectively. A gene was considered validated by qPCR analysis if revealed consistent trend with the nylon array data. Ath—A. thaliana, Bol—B. oleracea var. alboglabra
aab12039-sup-0002-AppendixS1.pdfPDF document101KAppendix S1. Protein alignment of PP2Cs homologues
aab12039-sup-0003-TableS1.pdfPDF document115KTable S1. Drought stress response gene homologues in B. oleracea identified in the macroarray experiment
aab12039-sup-0004-TableS2.pdfPDF document95KTable S2. Drought stress responsive genes in A. thaliana identified in the macroarray experiment
aab12039-sup-0005-TableS3.pdfPDF document13KTable S3. Gene names and accession numbers
aab12039-sup-0006-TableS4.pdfPDF document87KTable S4. Primers used for RLFP and SSR analysis
aab12039-sup-0007-TableS5.pdfPDF document95KTable S5. Primers used for qRT-PCR analysis

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