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Drosophila insulin-like peptide-6 (dilp6) expression from fat body extends lifespan and represses secretion of Drosophila insulin-like peptide-2 from the brain
Article first published online: 18 SEP 2012
© 2012 The Authors. Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Volume 11, Issue 6, pages 978–985, December 2012
How to Cite
Bai, H., Kang, P. and Tatar, M. (2012), Drosophila insulin-like peptide-6 (dilp6) expression from fat body extends lifespan and represses secretion of Drosophila insulin-like peptide-2 from the brain. Aging Cell, 11: 978–985. doi: 10.1111/acel.12000
- Issue published online: 15 NOV 2012
- Article first published online: 18 SEP 2012
- Accepted manuscript online: 31 AUG 2012 05:09AM EST
- Accepted for publication 30 July 2012
Fig. S1 dilp1, dilp2, dilp3 anddilp5 mRNA measured from fat body, midgut, ovary, brain and head carcass.
Fig. S2 DistribuLon of dilp6 reporter inadipose tissue and brain.
Fig. S3 Lifespan of dilp6over-expression using ubiquitous drivers: (A)Tub-GeneSwitch-Gal4, (B) da-GeneSwitch-Gal4, (C) neuronal Elav-GeneSwitch-Gal4.
Fig. S4 Lifespan of dilp6 silencingusing ubiquitous drivers: (A)Tub-GeneSwitch-Gal4; and fat body (B)S32-GeneSwitch-Gal4, (C)S106-GeneSwitch-Gal4.
Fig. S5 Specificity of anL-DILP2 and anL-DILP5 anLbodies in EIA.
Fig. S6 Upon 2% and 8% yeast diet,qRT-PCR verifies the inducLon of dilp6 byUAS-dilp6 (A,B), and dilp6 knock-down byRNAi (C).
Fig. S7 sNPF transcripts measured in flies withubiquitous and Lssue-specific dilp6overexpression.
Fig. S8 S106-gal4 does not induce transgene expression without RU.
Fig. S9 RU486 alone does not induce aging or metabolic phenotypes.
Fig. S10 Model for DILP6 to regulate lifespan by repressing DILP produced in the brain.
Fig. S11 Mortality rate (esLmated as–ln(ln(px)) for survival plots of text figure 2.
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