Fig. S1 Age-related gene expression changes and sample selection.

Fig. S2 Saturation curve of peak calling.

Fig. S3 The ChIP-Seq profiles around TSS region.

Fig. S4 Genome browser profiles of normalized macaque brain H3K4me2 ChIP-seq reads (left panels) and gluteal muscle ChIP-qPCR validation of increased H3K4me2 at PPARGC1B, SIRT1, NFKB2, PRDX2, IRS1/2 and WNT11 promoters (right panels) in the old (20, 21 and 22 years old) compared to the young (8, 9 and 9 years old) samples.

Fig. S5 Heatmap of the k-means clustering of genes that show age-related H3K4me2 changes at promoters (A) or enhancer-like regions (B).

Fig. S6 Robustness of the co-citation result over different gene sets or number of co-cited papers.

Fig. S7 Percentage of eICRGs co-cited with the indicated terms with at least 2 (A) or 3 (B) co-citation references.

Fig. S8 RT-qPCR analysis of the expression levels of H3K4 methyltransferases SETD7, DPY30 and MLL3 before or after DNA damage induced by 0.25 mg/mL 4NQO in HeLa cells.


Table S1. Summary of ChIP-seq samples.

Table S2. Genes whose promoter H3K4me2 levels increase in old versus young macaque brain samples determined by ChIP-seq were validated by ChIP-qPCR in young and old gluteal muscle samples.

Table S3. List of genes with promoter H3K4me2 levels that increase or decrease with age.

Table S4. List of promoter H3K4me2 cis-regulated genes (pCRGs) that show age-related increase (pICRG) or decrease (pDCRG).

Table S5. GO terms enriched among pI/DCRGs.

Table S6. List of terms used in co-citation analysis.

Table S7. List of enhancer-like regions with increased or decreased H3K4me2 levels with age.

Table S8. List of enhancer-like region H3K4me2 cis-regulated genes (eCRGs) that show age-related increase (eICRG) or decrease (eDCRG).

Table S9. GO terms enriched among eI/DCRGs determined by DAVID.

Table S10. qPCR primers for HeLa cells.

acel12007-sup-0003-DataS1.docWord document36K

Data S1. Experimental procedures.

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