These authors contributed equally to this work.
Stress-associated H3K4 methylation accumulates during postnatal development and aging of rhesus macaque brain
Article first published online: 18 OCT 2012
© 2012 The Authors Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Volume 11, Issue 6, pages 1055–1064, December 2012
How to Cite
Han, Y., Han, D., Yan, Z., Boyd-Kirkup, J. D., Green, C. D., Khaitovich, P. and Han, J.-D. J. (2012), Stress-associated H3K4 methylation accumulates during postnatal development and aging of rhesus macaque brain. Aging Cell, 11: 1055–1064. doi: 10.1111/acel.12007
- Issue published online: 15 NOV 2012
- Article first published online: 18 OCT 2012
- Accepted manuscript online: 15 SEP 2012 03:41AM EST
- Manuscript Accepted: 9 SEP 2012
- China Natural National Science Foundation. Grant Numbers: #30890033, 91019019
- Chinese Ministry of Science and Technology. Grant Numbers: #2011CB504206, Chinese Academy of Sciences, #KSCX2-EW-R-02, KSCX2-EW-J-15, XDA01010303
Fig. S1 Age-related gene expression changes and sample selection.
Fig. S2 Saturation curve of peak calling.
Fig. S3 The ChIP-Seq profiles around TSS region.
Fig. S4 Genome browser profiles of normalized macaque brain H3K4me2 ChIP-seq reads (left panels) and gluteal muscle ChIP-qPCR validation of increased H3K4me2 at PPARGC1B, SIRT1, NFKB2, PRDX2, IRS1/2 and WNT11 promoters (right panels) in the old (20, 21 and 22 years old) compared to the young (8, 9 and 9 years old) samples.
Fig. S5 Heatmap of the k-means clustering of genes that show age-related H3K4me2 changes at promoters (A) or enhancer-like regions (B).
Fig. S6 Robustness of the co-citation result over different gene sets or number of co-cited papers.
Fig. S7 Percentage of eICRGs co-cited with the indicated terms with at least 2 (A) or 3 (B) co-citation references.
Fig. S8 RT-qPCR analysis of the expression levels of H3K4 methyltransferases SETD7, DPY30 and MLL3 before or after DNA damage induced by 0.25 mg/mL 4NQO in HeLa cells.
Table S1. Summary of ChIP-seq samples.
Table S2. Genes whose promoter H3K4me2 levels increase in old versus young macaque brain samples determined by ChIP-seq were validated by ChIP-qPCR in young and old gluteal muscle samples.
Table S3. List of genes with promoter H3K4me2 levels that increase or decrease with age.
Table S4. List of promoter H3K4me2 cis-regulated genes (pCRGs) that show age-related increase (pICRG) or decrease (pDCRG).
Table S5. GO terms enriched among pI/DCRGs.
Table S6. List of terms used in co-citation analysis.
Table S7. List of enhancer-like regions with increased or decreased H3K4me2 levels with age.
Table S8. List of enhancer-like region H3K4me2 cis-regulated genes (eCRGs) that show age-related increase (eICRG) or decrease (eDCRG).
Table S9. GO terms enriched among eI/DCRGs determined by DAVID.
Table S10. qPCR primers for HeLa cells.
Data S1. Experimental procedures.
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