Aging leads to a programmed loss of brown adipocytes in murine subcutaneous white adipose tissue
Version of Record online: 24 OCT 2012
© 2012 The Authors Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Volume 11, Issue 6, pages 1074–1083, December 2012
How to Cite
Rogers, N. H., Landa, A., Park, S. and Smith, R. G. (2012), Aging leads to a programmed loss of brown adipocytes in murine subcutaneous white adipose tissue. Aging Cell, 11: 1074–1083. doi: 10.1111/acel.12010
- Issue online: 15 NOV 2012
- Version of Record online: 24 OCT 2012
- Accepted manuscript online: 30 SEP 2012 03:45AM EST
- Manuscript Accepted: 11 SEP 2012
|acel12010-sup-0001-DataS1.docx||Word document||128K||Data S1 Experimental procedures.|
Table S1 Adipogenesis PCR-array results.
Fig. S1 Ucp1 and cidea expression (relative to the endogenous control 36B4) in interscapular brown adipose tissue (BAT) or subcutaneous white adipose tissue (sWAT) isolated from 5 week old male mice. N = 3.
Fig. S2 Ucp1 and cidea expression (relative to endogenous control gene 36B4) in interscapular brown adipose tissue isolated from young (5 week) or old (11 month) male mice. N = 6–7.
Fig. S3 QPCR-confirmation of PCR array results for ppara, pparg2, and sfrp5 expression in subcutaneous white adipose tissue isolated from young (6 weeks) and old (1 year) male mice (relative to the endogenous control tbp; n = 3–4).
Fig. S4 (A) Plasma concentrations of adrenaline (white bars) and noradrenaline (black bars) are similar in young (11 weeks, gray bars) and old (11–12 months), black bars) mice. N = 4–6. (B–C) Plasma concentrations of triiodothyronine (B) and thyroxine (C) are elevated in old (12 months) vs. young (6 weeks) mice. N = 3–11; *P < 0.05, **P < 0.01.
Fig. S5 Volcano plot illustrating adipogenic gene expression in subcutaneous white adipose tissue (sWAT) in mice calorically restricted (CR) vs. age-matched (AM) ad libitum fed mice. Fold changes (CR vs. AM) are plotted on the x-axis, with genes altered >2-fold either to the left (decreased) or right (increased) of the gray vertical lines, and P-values are indicated on the y-axis, with significantly altered genes above the horizontal line indicating P = 0.05.
Fig. S6 Representative hematoxylin and eosin staining of epididymal white adipose tissue from mice calorically restricted for 1 year, with arrows pointing to multilocular cells.
Fig. S7 Brown-adipocyte related gene expression (relative to the endogenous control 36B4) in epididymal white adipose tissue isolated from 12–13 month old mice treated with vehicle (white bars) or β-3-adrenoreceptor agonist CL 316,243 (CL, black bars) for 7 days. *P < 0.05, ***P < 0.001 vs. veh.; n = 2–5.
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