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acel12010-sup-0001-DataS1.docxWord document128KData S1 Experimental procedures.
acel12010-sup-0002-TableS1-FigS1-S7.pdfapplication/PDF4148K

Table S1 Adipogenesis PCR-array results.

Fig. S1 Ucp1 and cidea expression (relative to the endogenous control 36B4) in interscapular brown adipose tissue (BAT) or subcutaneous white adipose tissue (sWAT) isolated from 5 week old male mice. N = 3.

Fig. S2 Ucp1 and cidea expression (relative to endogenous control gene 36B4) in interscapular brown adipose tissue isolated from young (5 week) or old (11 month) male mice. N = 6–7.

Fig. S3 QPCR-confirmation of PCR array results for ppara, pparg2, and sfrp5 expression in subcutaneous white adipose tissue isolated from young (6 weeks) and old (1 year) male mice (relative to the endogenous control tbp; n = 3–4).

Fig. S4 (A) Plasma concentrations of adrenaline (white bars) and noradrenaline (black bars) are similar in young (11 weeks, gray bars) and old (11–12 months), black bars) mice. N = 4–6. (B–C) Plasma concentrations of triiodothyronine (B) and thyroxine (C) are elevated in old (12 months) vs. young (6 weeks) mice. N = 3–11; *< 0.05, **< 0.01.

Fig. S5 Volcano plot illustrating adipogenic gene expression in subcutaneous white adipose tissue (sWAT) in mice calorically restricted (CR) vs. age-matched (AM) ad libitum fed mice. Fold changes (CR vs. AM) are plotted on the x-axis, with genes altered >2-fold either to the left (decreased) or right (increased) of the gray vertical lines, and P-values are indicated on the y-axis, with significantly altered genes above the horizontal line indicating = 0.05.

Fig. S6 Representative hematoxylin and eosin staining of epididymal white adipose tissue from mice calorically restricted for 1 year, with arrows pointing to multilocular cells.

Fig. S7 Brown-adipocyte related gene expression (relative to the endogenous control 36B4) in epididymal white adipose tissue isolated from 12–13 month old mice treated with vehicle (white bars) or β-3-adrenoreceptor agonist CL 316,243 (CL, black bars) for 7 days. *< 0.05, ***< 0.001 vs. veh.; n = 2–5.

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