The authors have declared that no conflicts of interest exists.
Original Article
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Age and gender effects on DNA strand break repair in peripheral blood mononuclear cells
Article first published online: 26 NOV 2012
DOI: 10.1111/acel.12019
© 2012 The Authors Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland
Additional Information
How to Cite
Garm, C., Moreno-Villanueva, M., Bürkle, A., Petersen, I., Bohr, V. A., Christensen, K. and Stevnsner, T. (2013), Age and gender effects on DNA strand break repair in peripheral blood mononuclear cells. Aging Cell, 12: 58–66. doi: 10.1111/acel.12019
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The authors have declared that no conflicts of interest exists.
Publication History
- Issue published online: 17 JAN 2013
- Article first published online: 26 NOV 2012
- Accepted manuscript online: 22 OCT 2012 10:16AM EST
- Manuscript Accepted: 7 OCT 2012
Funded by
- VELUX Foundation
- Ausschuss für Forschungsfragen
- University of Konstanz
- Danish Agency for Science, Technology and Innovation
References
- , , , , , , , , , , , , , (2002) Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX. Proc. Natl Acad. Sci. USA 99, 8173–8178.
- , , , , , , , , , , , , , , , , , , , , , , , , (2010) Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial. Mutagenesis 25, 113–123.
- , , , , , , , , , , , , , , , , , , , , , , , , , , , (2012) Age-related somatic structural changes in the nuclear genome of human blood cells. Am. J. Hum. Genet. 90, 217–228.
- , , , , , , , , , (1999) Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC). Exp. Gerontol. 34, 645–658.
- , , , , , , (2002) Hand grip strength: a phenotype suitable for identifying genetic variants affecting mid- and late-life physical functioning. Genet. Epidemiol. 23, 110–122.
- , , (2001) Dietary probiotic supplementation enhances natural killer cell activity in the elderly: an investigation of age-related immunological changes. J. Clin. Immunol. 21, 264–271.
- (2010) DNA damage and base excision repair in mitochondria and their role in aging. J Aging Res. 2011, 257093.
- (1992) Role of free radicals in aging and disease. Ann. N. Y. Acad. Sci. 673, 126–141.Direct Link:
- , , (2006) DNA oxidative damage and strand breaks in young healthy individuals: a gender difference and the role of life style factors. Free Radic Res. 40, 707–714.
- , , , , , , (2007) Age-related increases in DNA repair and antioxidant protection: a comparison of the Boyd Orr Cohort of elderly subjects with a younger population sample. Age Ageing 36, 521–526.
- , , , , , (2011) The association of APOE genotype with cognitive function in persons aged 35 years or older. PLoS ONE 6, e27415.
- , , (2011) The role of homologous recombination in radiation-induced double-strand break repair. Radiother. Oncol. 101, 7–12.
- , , (2011) DNA repair deficiency in neurodegeneration. Prog. Neurobiol. 94, 166–200.
- , , (2007) Adjusting batch effects in microarray expression data using empirical Bayes methods. Biostatistics 8, 118–127.
- , , , , , , , , , , , , , (2006) Decreased expression of DNA repair proteins Ku70 and Mre11 is associated with aging and may contribute to the cellular senescence. Exp. Mol. Med. 38, 686–693.
- , , , (2004) Use of the tail moment of the lymphocytes to evaluate DNA damage in human biomonitoring studies. Toxicol. Sci. 81, 121–132.
- , , , , , , , , (2010) Tackling the widespread and critical impact of batch effects in high-throughput data. Nat. Rev. Genet. 11, 733–739.
- , , (2008) DNA double-strand breaks: a potential causative factor for mammalian aging? Mech. Ageing Dev. 129, 416–424.
- , , , , , , (2003) Base excision repair activities required for yeast to attain a full chronological life span. Aging Cell 2, 93–104.
- , (2008) Declining cellular fitness with age promotes cancer initiation by selecting for adaptive oncogenic mutations. Biochim. Biophys. Acta 1785, 1–11.
- , , , (1991) Gender differences in age-related decline in DNA double-strand break damage and repair in lymphocytes. Ann. Hum. Biol. 18, 405–415.
- , , , , (2009) Base excision repair of oxidative DNA damage and association with cancer and aging. Carcinogenesis 30, 2–10.
- (2006) Assessment of reference values for DNA damage detected by the comet assay in human blood cell DNA. Mutat. Res. 612, 84–104.
- , , , , , , (2009) A modified and automated version of the ‘Fluorimetric Detection of Alkaline DNA Unwinding’ method to quantify formation and repair of DNA strand breaks. BMC Biotechnol. 9, 39.
- , , , , , , , , (2011) The automated FADU-assay, a potential high-throughput in vitro method for early screening of DNA breakage. ALTEX 28, 295–303.
- , , , , (1996) The effect of donor age on the processing of UV-damaged DNA by cultured human cells: reduced DNA repair capacity and increased DNA mutability. Mutat. Res. 364, 117–123.
- , , , , (2001) Radiation sensitivity of lymphocytes from healthy individuals and cancer patients as measured by the comet assay. Radiat. Environ. Biophys. 40, 83–89.
- , , , (2008) An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells. Nat. Protoc. 3, 1187–1193.
- , , , , , (2003) Age-related increases in plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in elderly subjects. Clin. Biochem. 36, 397–400.
- , , , , , , (2002) Rapid assessment of repair of ultraviolet DNA damage with a modified host-cell reactivation assay using a luciferase reporter gene and correlation with polymorphisms of DNA repair genes in normal human lymphocytes. Mutat. Res. 509, 165–174.
- , (2002) Non-homologous DNA end joining in the mature rat brain. J. Neurochem. 80, 949–959.
- , , , , (2003) DNA damage recognition and repair capacities in human naive and memory T cells from peripheral blood of young and elderly subjects. Mech. Ageing Dev. 124, 517–524.
- , , (2008) Regulation of DNA double-strand break repair pathway choice. Cell Res. 18, 134–147.
- , , , (2011) The Danish Twin Registry. Scand. J. Public Health 39, 75–78.
- , , , , , , , , , (2011) DNA damage and nucleotide excision repair capacity in healthy individuals. Environ. Mol. Mutagen. 52, 511–517.
- , , , , , , (2008) Age, sex, and race influence single-strand break repair capacity in a human population. Free Radic. Biol. Med. 45, 1631–1641.
- , , , , , , , , (2012) Oxidative damage to DNA and single strand break repair capacity: relationship to other measures of oxidative stress in a population cohort. Mutat. Res. 736, 93–103.
- , , , , , (2011) Sex differences in susceptibility to PAHs is an intrinsic property of human lung adenocarcinoma cells. Lung Cancer. 71, 264–270.
- , (2006) DNA double strand break repair in brain: reduced NHEJ activity in aging rat neurons. Neurosci. Lett. 393, 18–22.
- , , , , (1993) DNA repair and aging in basal cell carcinoma: a molecular epidemiology study. Proc. Natl Acad. Sci. USA 90, 1614–1618.
- , , , , , , , (2004) Control of sister chromatid recombination by histone H2AX. Mol. Cell 16, 1017–1025.
- , , (2010) Focus on histone variant H2AX: to be or not to be. FEBS Lett. 584, 3717–3724.
- , , , , , , , , , , , , , , (2006) Age-dependent oxidative stress-induced DNA damage in Down's lymphocytes. Biochem. Biophys. Res. Commun. 345, 726–733.

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