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acel12031-sup-0001-SupplementaryMaterial-TableS1-S3.docWord document229K

Table S1 Disaccharide composition and sulfate distribution within HS extracted from OECs isolated from cord blood (n = 5) and peripheral blood samples of young (n = 5) and old (n = 4) healthy subjects.

Table S2 Pearson's correlation coefficient between measures of 6-O-sulfated disaccharide residues and OEC transwell migration.

Table S3 Details of primer sequences used for conventional RT-PCR and Quantitative real time RT-PCR

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acel12031-sup-0002-FigS1.tifimage/tif912KFig. S1 Outgrowth endothelial cells (OECs) from adult peripheral blood.
acel12031-sup-0003-FigS2.tifimage/tif2503KFig. S2 OEC proliferation and apoptosis are not significantly altered with age.
acel12031-sup-0004-FigS3.tifimage/tif1156KFig. S3 The tube-forming potential of OECs in growth factor reduced Matrigel is not significantly impaired with age.
acel12031-sup-0005-FigS4.tifimage/tif2643KFig. S4 Comparison of the expression levels of CXCR4 and VEGFR-2 from OECs isolated from cord blood and peripheral blood of young and old subjects.
acel12031-sup-0006-FigS5.tifimage/tif584KFig. S5 Representative reverse phase (RP)-HPLC chromatogram of AMAC-labelled HS disaccharide subunits isolated from OECs.
acel12031-sup-0007-FigS6.tifimage/tif940KFig. S6 Age-associated changes in O-sulfate distribution within OEC HS.
acel12031-sup-0008-FigS7.tifimage/tif2837KFig. S7 Comparison of the molecular weight of HS isolated from OECs of cord blood and peripheral blood samples of young and old subjects.
acel12031-sup-0009-FigS8.tifimage/tif2873KFig. S8 Correlation between the overall extent of 6-O-sulfation of glucosamine residues within HS chains on the cell surface of OECs and cell migration toward.
acel12031-sup-0010-FigS9.tifimage/tif2897KFig. S9 Correlation between the percentage expression of the of the disaccharide UA-GlcNS[6S] within HS chains on the cell surface and OEC migration toward (A) SDF-1 and (B) VEGF.
acel12031-sup-0011-FigS10.tifimage/tif445KFig. S10 Correlation between the percentage expression of the of the disaccharide UA-GlcNAc[6S] within HS chains on the cell surface and OEC migration toward (A) SDF-1 and (B) VEGF.
acel12031-sup-0012-FigS11.tifimage/tif564KFig. S11 Comparison of the expression levels of SULF-1 and SULF-2 from OECs isolated from cord blood (n = 4) and peripheral blood of young (n = 4) and old (n = 4) subjects.

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