acel12045-sup-0001-FigS1.tifimage/tif4767KFig. 1 Regional differences in basement membrane thickness with age. (A–F) Electron micrographs of basement membranes (arrows) of capillaries from the brains of 2- (A and D), 7- (B and E), and 23- (C and F)-month-old mice did not show differences in thickness in the striatum with increasing age (A–C). By contrast, there was a significant increase in basement membrane thickness with age in capillaries in the frontoparietal cortex (D–F). Original magnification = ×50 000.
acel12045-sup-0002-FigS2.tifimage/tif5343KFig. 2 Increased perlecan expression with age. (A–L) Photomicrographs of perlecan immunoreactivity in blood vessels in the cortex (A–C), hippocampus (D–F), striatum (G–I), and thalamus (J–L) of 2- (A, D, G, J), 7- (B, E, H, K), and 23- (C, F, I, L)-month-old mice.
acel12045-sup-0003-FigS3.tifimage/tif398KFig. 3 Arterial blood vessel density by brain region and age. (A and B) Mean (± S.E.M.) density of potato lectin staining of arteries in the brains of 7- (A) and 20-month-old (B) mice. No differences were noted in percent area coverage of lectin between the cortex, striatum, hippocampus, or thalamus in either young or aged mice.
acel12045-sup-0004-FigS4.tifimage/tif2398KFig. 4 Vascular anatomy of the thalamus and hippocampus of the adult mouse brain. (A and B) Anterior (A) and lateral view (B) showing the posterior cerebral artery blood supply (purple) to the thalamus (green) and the hippocampus (blue) in the mouse. Yellow arrows indicate the flow of solutes moving from the thalamus to the hippocampus. Vasculature was imaged with micro-CT and registered to MRI of brain structures. Posterior cerebral artery, thalamus, and hippocampus segmentations and 3D images made using Display software (Montreal Neurological Institute Brain Imaging Centre, Montreal, Canada).

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