Primary human muscle precursor cells obtained from young and old donors produce similar proliferative, differentiation and senescent profiles in culture
Article first published online: 17 MAR 2013
© 2013 The Authors Aging Cell © 2013 John Wiley & Sons Ltd and the Anatomical Society
Volume 12, Issue 3, pages 333–344, June 2013
How to Cite
Alsharidah, M., Lazarus, N. R., George, T. E., Agley, C. C., Velloso, C. P. and Harridge, S. D. R. (2013), Primary human muscle precursor cells obtained from young and old donors produce similar proliferative, differentiation and senescent profiles in culture. Aging Cell, 12: 333–344. doi: 10.1111/acel.12051
- Issue published online: 13 MAY 2013
- Article first published online: 17 MAR 2013
- Accepted manuscript online: 1 FEB 2013 10:25AM EST
- Manuscript Accepted: 16 JAN 2013
Fig. S1 (a) Time course of MyoD + MHC expression, in human MPCs obtained from young (n = 5) and elderly (n = 4) subjects. (b) Time course of MyoD + MHC expression, in senescent cells (n = 5) vs. non-senescent cells (n = 5). Cells were either differentiated at early passage or at several time points during the culture period (16 and 28 days, n = 5) until they reached replicative senescence. Data are mean value ± SD.
Fig. S2 Images of cells taken from (a) young (from a male subject aged 25), (b) elderly (from a male subject aged 80) and (c) senescent MPCs stained for MyoD (green) + MHC (red) and Hoechst (blue) after 7 days in differentiation medium. Scale bar = 20 μm. Young and elderly cells were similar in terms of MHC and MyoD expression. There was a decrease in the onset of MHC and MyoD expression when cells reached senescence.
Table S1 Primary antibodies used.
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