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acel12063-sup-0001-FigS1-S7.pdfapplication/PDF280K

Fig. S1 The process of loading young adult worms into PDMS chamber.

Fig. S2 Worms living in PDMS chambers have a similar physiological growth as worms living on NGM plates.

Fig. S3 The reproducibility of worm lifespan assays upon RNAi of aging-related genes.

Fig. S4 The reproducibility of worm lifespan assays in glucose medium.

Fig. S5 Age-related changes in fluorescence intensities.

Fig. S6 Oxidative stress tolerance assay in WormFarm.

Fig. S7 Comparison of survival rates calculated from our computer program with or without considering clustered worms to those from manual counting.

acel12063-sup-0002-MovieS1.avivideo/avi2331KVideo S1 Load 2% pluronic solution to make the channel lubricant.
acel12063-sup-0003-MovieS2.avivideo/avi2621KVideo S2 Load young adult worms into PDMS chamber.
acel12063-sup-0004-MovieS3.avivideo/avi6531KVideo S3 Wash off the offspring of adult worms in PDMS chamber.
acel12063-sup-0005-MovieS4.avivideo/avi1779KVideo S4 Movie of 5-day old worms in PDMS chamber (upper panel, sptf-3 RNAi; lower panel, Ctrl RNAi).
acel12063-sup-0006-MovieS5.avivideo/avi1805KVideo S5 Movie of 5-day old worms in PDMS chamber (upper panel, age-1 RNAi; lower panel, Y82E9BR.3 RNAi).
acel12063-sup-0007-SupplementaryMaterial.docWord document534K

Note S1 Formulas for calculating worm features/phenotypes.

Note S2 WormFarm video analysis procedure.

acel12063-sup-0008-TableS1-S6.xlsxapplication/msexcel36K

Table S1 Log-rank test results between automatically counted mean lifespans of different repeats in RNAi experiments.

Table S2 Log-rank test results between automatically counted mean lifespans of different repeats of glucose diet test.

Table S3 Significances of difference between the RNAi groups for worm length, width, area and motility as determined by Student's t-test.

Table S4 Raw data for lifespan assays.

Table S5 Raw data for mitochondrial fluorescent density assay.

Table S6 Raw data of average values of worm phenotypic features used in principal component analysis.

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