acel12087-sup-0001-FigureS1.TIFimage/tif270KFig. S1 Changes of SA-β-gal activities and intracellular ROS levels during replicative senescence. HDFs were continuously subcultured in DMEM medium containing 10% FBS and their PD and DT were recorded. (A) Representative images of SA-β-gal positive cell populations are shown. (B) Representative DCF-stained cell population profiles are shown.
acel12087-sup-0002-FigureS2.TIFimage/tif125KFig. S2 Expression shift of MMPs and TIMPs during replicative senescence. Gene expression profiles of MMPs and TIMPs are shown from cDNA microarray analysis.
acel12087-sup-0003-FigureS3a.TIFimage/tif366KFig. S3 Representative schematic pathways are obtained from KEGG database. (A) RIG-I-receptor signaling pathway. (B) Toll-like receptor signaling pathway. (C) Lysosomal pathway. (D) p53 pathway. The genes found in the given module are indicated by red star.
acel12087-sup-0004-FigureS4.TIFimage/tif244KFig. S4 Hypothesis of possible action modes of senescent cell in tissue. Three possible action modes are drawn based on the differential expressions of interleukins and their receptors, and types of major cell population in tissues. Four different action groups of interleukin family genes were classified based on the result of figure 5A and described on the bottom.
acel12087-sup-0005-FigureS5.tifimage/tif87KFig. S5 Expressions of senescence-associated factors controlling cell cycle progression during replicative senescence. Cell lysate (30 μg) for each DT cell was subjected to Western blot analysis. Antibodies for PCNA (NCL-PCNA, Leica Biosystems, Newcastle, UK), P21 (sc-379, Santa Cruz Biotechnology, CA), P16 (Cat. 554079, BD Biosciences, CA), and p53 (sc-126, Santa Cruz Biotechnology, CA) were used.
acel12087-sup-0007-TableS1.xlsxapplication/msexcel80KTable S1 Genes belonged to four different modules shown in the process of HDF replicative senescence.
acel12087-sup-0006-FigureLegends.docxWord document18K 

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