SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
acel12100-sup-0001-FigS1-S5-TableS1-S5.docWord document1183K

Fig. S1 Western blot showing CRY protein levels in head extracts of 5 and 50 day-old tim>CRY flies and controls at ZT 4 and 16. LC represents the loading control. CRY levels were lower in the morning (ZT 4) than at night (ZT 16) in both young and old tim>CRY flies, suggesting light sensitivity of ectopic CRY.

Fig. S2 Average daily activity profiles of 5, 35, and 50 day-old males of the indicated genotypes in LD (12:12). Each bar represents daily activity counts in a 15 min bin averaged for 3 days.

Fig. S3 Analysis of the rate of senescence in males of the indicated genotypes for the following parameters A) FFT, B) % Rhythmicity, and C) Vertical mobility. For each parameter, the graph on the left side shows average values obtained on days 5, 35, and 50, while bar diagrams on the right indicate the time required for function to decline to 50% and 75% of its peak value, interpolated from second-order polynomial curves.

Fig. S4 Average daily activity profiles of 5, 35, and 50 day-old males of the indicated genotypes in LD (12:12). Each bar represents daily activity counts in a 15 min bin averaged for 3 days.

Fig. S5 Analysis of the rate of senescence in males of the indicated genotypes for the following parameters A) FFT, B) % Rhythmicity, and C) Vertical mobility. For each parameter, the graph on the left side shows average values obtained on days 5, 35, and 50, while bar diagrams on the right indicate the time required for function to decline to 50% and 75% of its peak value, interpolated from second-order polynomial curves.

Table S1 Statistical analysis of gene expression (qPCR) and protein (Western Blotting) data by two-way ANOVA with Bonferroni's post-hoc test.

Table S2 Statistical analysis of gene expression (qPCR) data in heads of 50 day-old flies by two-way ANOVA with Bonferroni's post-hoc test.

Table S3 Locomotor activity analysis of flies overexpressing CRY.

Table S4 Statistical analysis of Rapid Iterative Negative Geotaxis (RING) data by two-way ANOVA with Bonferroni's post-hoc test.

Table S5 Median lifespan (days) is shown for indicated genotypes with n = sample size.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.