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Fig. S1 SS-31 is rapidly elevated in skeletal muscle and declines to 10% of its maximum value after 16 h.

Fig. S2 ETC complex protein expression was not different one hour after injection of 3 mg kg−1 SS-31 in old mice.

Fig. S3 Representative fully relaxed spectra from SS-31-treated and control old mice illustrate an increase in relative peak area of PCr to γATP.

Fig. S4 Daily SS-31 treatment did not affect voluntary running distance in old mice. Mean ± SEM. = 6–7 per group.

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