The human ΔNp53 isoform triggers metabolic and gene expression changes that activate mTOR and alter mitochondrial function
Article first published online: 25 JUN 2013
© 2013 The Anatomical Society and John Wiley & Sons Ltd
Volume 12, Issue 5, pages 863–872, October 2013
How to Cite
Lin, S.-C., Karoly, E. D. and Taatjes, D. J. (2013), The human ΔNp53 isoform triggers metabolic and gene expression changes that activate mTOR and alter mitochondrial function. Aging Cell, 12: 863–872. doi: 10.1111/acel.12108
- Issue published online: 11 SEP 2013
- Article first published online: 25 JUN 2013
- Accepted manuscript online: 4 JUN 2013 07:22AM EST
- Manuscript Accepted: 26 MAY 2013
- NCI. Grant Number: CA127364
- American Cancer Society
- Ellison Medical Foundation
- NIH. Grant Number: R03 AG035228
Fig. S1 Schematic of ΔNp53:WTp53.
Fig. S2 Representative Western blot and cell cycle data.
Fig. S3 Assessment of apoptosis, growth, and senescence
Fig. S4 GSEA of microarray data.
Fig. S5 Venn diagrams of gene expression data.
Fig. S6 qRT–PCR validation of microarray data.
Fig. S7 qRT–PCR data from Saos-2 cells.
Fig. S8 Evaluation of WTp53:WTp53 tetramers.
Fig. S9 qRT–PCR data comparing ΔNp53:WTp53 with ΔNp53 and WTp53 coexpressed at equal levels.
Fig. S10 Comparison of ΔNp53-induced gene expression changes in H1299 cells.
Fig. S11 Additional data for mitochondrial function.
Fig. S12 Additional data for mTOR pathway.
Table S1 Genes up-regulated 1.5-fold or greater in H1299 cells expressing ΔNp53:WTp53, compared with WTp53.
Table S2 Genes down-regulated 1.5-fold or greater in H1299 cells expressing ΔNp53:WTp53, compared with WTp53.
Table S3 Overview of metabolomics data.
Table S4 Metabolomics data.
Table S5 Summary of metabolic changes induced by WTp53.
Table S6 Pathway analysis of gene expression data: GO Processes and GO Metabolic Networks.
Table S7 Pathway analysis of gene expression data: GO Pathways.
Table S8 Pathway analysis of Metabolomics data.
Table S9 Merged Pathway analysis of gene expression and metabolomics data.
Table S10 Primer sequences used for cloning and qRT–PCR.
Data S1 Experimental procedures.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.