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acel12110-sup-0001-FigS1.pdfapplication/PDF13158KFig. S1 (A–B) Age-synchronized wt or glp-1 animals were subjected to HS (37 °C) on (A) day 1 or (B) day 2 of adulthood and survival was assayed by manual scoring at the indicated times. (C) Quantification of hsp-70 (left) and hsp-16.11 (right) mRNA levels from age-synchronized gon-2 mutant animals untreated or subjected to HS (90 min at 37°C) as in Fig. 1C. (D) Extracts of animals grown as described in the legend to Fig. 1E were probed with anti-GFP (bottom) and anti-paramyosin (top) antibodies. (E) Confocal images of age-synchronized wt or glp-1 animals expressing pC12C8.1::mCherry, following HS (09 min at 37°C) on either day 1 or day 3 of adulthood. (F) Confocal images of untreated age-synchronized wt or glp-1 animals expressing phsp-16.2:GFP on either day 1 or day 3 of adulthood. (G) Confocal images of age-synchronized wt or glp-1 animals expressing phsp-16.2::GFP. Animals were grown at 15°C and subjected to HS (90 min at 34°C) on either day 2 or day 5 of adulthood.
acel12110-sup-0002-FigS2.pdfapplication/PDF21700KFig. S2 (A) Images of animals described in the legend to Fig. 2A. (B) Age-synchronized wt animals expressing phsp-16.2::GFP were grown on the indicated RNAi-expressing bacteria and subjected to HS (90 min at 37°C) on day 1 of adulthood. (C) Age-synchronized mutant animals (as indicated) were exposed to a HS (6h at 37°C) at day 2 of adulthood and survival was assayed. (D-G) Quantification of (D) hsp-16.2 (E) hsp-16.11 (F) C12C8.1 and (G) F44E5.4 mRNA levels from age-synchronized glp-1 mutant animals grown on the indicated RNAi-expressing bacteria and subjected to HS (90 min at 37°C) on day 2 of adulthood.
acel12110-sup-0003-FigS3.pdfapplication/PDF6749KFig. S3 Extracts of age-synchronized (day 2 of adulthood) Q35 or Q35;glp-1 animals were probed with anti-GFP (bottom) and anti-paramyosin (top) antibodies.
acel12110-sup-0004-FigS4.pdfapplication/PDF261KFig. S4 Age-synchronized unc-52(ts);glp-1 animals were grown on the indicated RNAi-expressing bacteria and scored for the percent of stiff-body paralyzed animals on day 4 of adulthood.
acel12110-sup-0005-TableS1.pdfapplication/PDF27KTable S1 Effect of reproduction mutations on HS survival.
acel12110-sup-0006-TableS2.pdfapplication/PDF16KTable S2 List of strains and abbreviations used in this work.
acel12110-sup-0007-TableS3.pdfapplication/PDF11KTable S3 List of crosses used in this work.

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