Senescence-associated lncRNAs: senescence-associated long noncoding RNAs
Version of Record online: 14 JUL 2013
Published 2013. This article is a U.S. Government work and is in the public domain in the USA.
Volume 12, Issue 5, pages 890–900, October 2013
How to Cite
Abdelmohsen, K., Panda, A., Kang, M.-J., Xu, J., Selimyan, R., Yoon, J.-H., Martindale, J. L., De, S., Wood, W. H., Becker, K. G. and Gorospe, M. (2013), Senescence-associated lncRNAs: senescence-associated long noncoding RNAs. Aging Cell, 12: 890–900. doi: 10.1111/acel.12115
- Issue online: 11 SEP 2013
- Version of Record online: 14 JUL 2013
- Accepted manuscript online: 13 JUN 2013 02:30AM EST
- Manuscript Accepted: 5 JUN 2013
- post-transcriptional gene regulation;
- senescence-associated gene expression patterns;
Noncoding RNAs include small transcripts, such as microRNAs and piwi-interacting RNAs, and a wide range of long noncoding RNAs (lncRNAs). Although many lncRNAs have been identified, only a small number of lncRNAs have been characterized functionally. Here, we sought to identify lncRNAs differentially expressed during replicative senescence. We compared lncRNAs expressed in proliferating, early-passage, ‘young’ human diploid WI-38 fibroblasts [population doubling (PDL) 20] with those expressed in senescent, late-passage, ‘old’ fibroblasts (PDL 52) by RNA sequencing (RNA-Seq). Numerous transcripts in all lncRNA groups (antisense lncRNAs, pseudogene-encoded lncRNAs, previously described lncRNAs and novel lncRNAs) were validated using reverse transcription (RT) and real-time, quantitative (q)PCR. Among the novel senescence-associated lncRNAs (SAL-RNAs) showing lower abundance in senescent cells, SAL-RNA1 (XLOC_023166) was found to delay senescence, because reducing SAL-RNA1 levels enhanced the appearance of phenotypic traits of senescence, including an enlarged morphology, positive β-galactosidase activity, and heightened p53 levels. Our results reveal that the expression of known and novel lncRNAs changes with senescence and suggests that SAL-RNAs play direct regulatory roles in this important cellular process.