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Fig. S1 Antiproliferative effect of abrogation of DGCR8 in fibroblasts. (A) QPCR analysis of the silencing efficiency of the indicated shRNA vectors, transcript levels for Dicer, Drosha or DGCR8 was normalized to the levels in empty vector-infected cells. (B) Long term proliferation of IMR-90 human fibroblasts retrovirally infected with two shRNAs against DGCR8. (C) Crystal violet staining of IMR-90 human fibroblasts retrovirally infected as in A. (D) Growth curve of IMR-90 human fibroblasts infected with two independent shRNAs against Dicer or Drosha, or an empty vector.

Fig. S2. (A) Western blot analysis of the indicated proteins in IMR90 fibroblasts, after infection with shRNA vectors. (B) Western blot analysis of the indicated proteins in MEF of the indicated genotypes, after infection with shRNA vectors. Erk, total Erk1/2 protein; P-Erk, phosphorylated Erk1/2.

Fig. S3 (A) Representative images of DAPI staining of nuclei from MEFs of the indicated genotype, top, or IMR-90 human fibroblasts, bottom, expressing the indicated shRNA vectors. (B) Percentage of nuclei positive for gamma-H2AX staining in IMR-90 and BJ fibroblasts. BJ fibroblasts irradiated with 10 Gy of gamma irradiation are shown as a positive control.

Fig. S4 Schematic representation of target sequences for the indicated miRNAs in the 3′UTR region of human p21CIP1, showing their complementarity to seed sequences of the miRNAs.

Fig. S5 (A) Relative cell number estimated by crystal violet staining of HCT-116 and HCT-116 p53KO cells after silencing of DGCR8. (B) Western blot analysis of the indicated proteins in U2OS or 293T cells, after infection and selection (U2OS) or transient transfection (293T) with the indicated shRNA vectors.

Fig. S6 Cell-cycle regulators with differential expression in shDGCR8 IMR90 cells.

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