These authors contributed equally to this research.
SIRT1 but not its increased expression is essential for lifespan extension in caloric-restricted mice
Article first published online: 19 NOV 2013
© 2013 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
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How to Cite
Mercken, E. M., Hu, J., Krzysik-Walker, S., Wei, M., Li, Y., McBurney, M. W., de Cabo, R. and Longo, V. D. (2013), SIRT1 but not its increased expression is essential for lifespan extension in caloric-restricted mice. Aging Cell. doi: 10.1111/acel.12151
- Article first published online: 19 NOV 2013
- Accepted manuscript online: 14 AUG 2013 05:06AM EST
- Manuscript Accepted: 8 AUG 2013
- NIH/NIA . Grant Numbers: AG20642, AG025135, AG034906
Fig. S1 Parametric analysis of gene-set enrichment (PAGE) was performed on microarray data from SIRT1+/+, SIRT1+/− and SIRT1−/− mice fed ad libitum (AL) or subjected to 40% CR.
Fig. S2 Genes affected by CR in a SIRT1 gene-dose dependent manner. (A) IRS2 GCK, and MUP1 mRNA levels were measured by RT-qPCR in liver homogenates from ad libitum (AL) and 40% CR-fed SIRT1+/+, SIRT1+/− and SIRT1−/− mice. n = 3 for all groups (*p < 0.05 compared to SIRT1+/+).
Table S1 Numbers and survival characteristics of SIRT1+/+, SIRT1+/− and SIRT1−/− ad libitum (AL) and calorie-restricted (CR) mice.
Table S2 Significance for all pairwise comparisons (diet, genotype and sex) using log rank test.
Table S3 Z-scores of top 100 genes altered by CR and sorted against SIRT1−/− mice.
Table S4 List of genes affected by CR in a SIRT1-independent manner.
Table S5 RT-qPCR validation of genes affected by CR in a SIRT1-independent manner.
Table S6 Pathologies and abnormalities in old mice (> 24 months).
Table S7 RT-qPCR validation of microarray data.
Table S8 Primer sequences used for quantitative PCR analysis.
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