These authors contributed equally to this research.
SIRT1 but not its increased expression is essential for lifespan extension in caloric-restricted mice
Version of Record online: 19 NOV 2013
© 2013 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 13, Issue 1, pages 193–196, February 2014
How to Cite
Mercken, E. M., Hu, J., Krzysik-Walker, S., Wei, M., Li, Y., McBurney, M. W., de Cabo, R. and Longo, V. D. (2014), SIRT1 but not its increased expression is essential for lifespan extension in caloric-restricted mice. Aging Cell, 13: 193–196. doi: 10.1111/acel.12151
- Issue online: 16 JAN 2014
- Version of Record online: 19 NOV 2013
- Accepted manuscript online: 14 AUG 2013 05:06AM EST
- Manuscript Accepted: 8 AUG 2013
- NIH/NIA . Grant Numbers: AG20642, AG025135, AG034906
Fig. S1 Parametric analysis of gene-set enrichment (PAGE) was performed on microarray data from SIRT1+/+, SIRT1+/− and SIRT1−/− mice fed ad libitum (AL) or subjected to 40% CR.
Fig. S2 Genes affected by CR in a SIRT1 gene-dose dependent manner. (A) IRS2 GCK, and MUP1 mRNA levels were measured by RT-qPCR in liver homogenates from ad libitum (AL) and 40% CR-fed SIRT1+/+, SIRT1+/− and SIRT1−/− mice. n = 3 for all groups (*p < 0.05 compared to SIRT1+/+).
Table S1 Numbers and survival characteristics of SIRT1+/+, SIRT1+/− and SIRT1−/− ad libitum (AL) and calorie-restricted (CR) mice.
Table S2 Significance for all pairwise comparisons (diet, genotype and sex) using log rank test.
Table S3 Z-scores of top 100 genes altered by CR and sorted against SIRT1−/− mice.
Table S4 List of genes affected by CR in a SIRT1-independent manner.
Table S5 RT-qPCR validation of genes affected by CR in a SIRT1-independent manner.
Table S6 Pathologies and abnormalities in old mice (> 24 months).
Table S7 RT-qPCR validation of microarray data.
Table S8 Primer sequences used for quantitative PCR analysis.
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