The conserved Mediator subunit MDT-15 is required for oxidative stress responses in Caenorhabditis elegans
Version of Record online: 18 SEP 2013
© 2013 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 13, Issue 1, pages 70–79, February 2014
How to Cite
Goh, G. Y. S., Martelli, K. L., Parhar, K. S., Kwong, A. W. L., Wong, M. A., Mah, A., Hou, N. S. and Taubert, S. (2014), The conserved Mediator subunit MDT-15 is required for oxidative stress responses in Caenorhabditis elegans. Aging Cell, 13: 70–79. doi: 10.1111/acel.12154
- Issue online: 16 JAN 2014
- Version of Record online: 18 SEP 2013
- Accepted manuscript online: 19 AUG 2013 10:23PM EST
- Manuscript Accepted: 2 AUG 2013
- NIH Office of Research Infrastructure Programs. Grant Number: P40 OD010440
- CIHR. Grant Number: MOP-93713
- UBC and CFRI scholarships
Fig. S1 SKN-1 and DAF-16 expression and localization are unaffected by mdt-15 depletion or mutation.
Fig. S2 mdt-15 is required for arsenite gene inductions in L4 and adult worms.
Fig. S3 MDT-15 is required to upregulate SKN-1 targets upon loss of wdr-23.
Fig. S4 Expression controls for yeast-two-hybrid fusion proteins.
Fig. S5 The tm2182 mutation is an in-frame deletion and the resulting MDT-15 protein is expressed in vivo and transcriptionally active in yeast.
Fig. S6 Requirement of mdt-15 for gene expression and longevity in two daf-2 mutants.
Fig. S7 fat-6(tm331); fat-7(wa36) double mutants are not sensitive to tBOOH and fat-6 RNAi causes developmental defects.
Fig. S8 mdt-15 is required to induce several tBOOH responsive genes.
Table S1 Identities of genes downregulated in mdt-15(RNAi) worms and induced by oxidative stress.
Table S2 Survival of mutant strains on 5 mM sodium arsenite.
Table S3 Survival of mutant strains on 6 mM tBOOH.
Table S4 Survival of RNAi-treated worms on 6 mM tBOOH.
Table S5 Survival of PUFA-treated worms on 6 mM tBOOH.
Table S6 Lifespans of daf-2(e1370) mutants on control and mdt-15 RNAi.
Table S7 Lifespans of daf-2(e1368) mutants on control and mdt-15 RNAi.
Table S8 List of worm strains.
Table S9 List of qPCR primers.
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