Integrin-linked kinase modulates longevity and thermotolerance in C. elegans through neuronal control of HSF-1
Article first published online: 9 JAN 2014
© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 13, Issue 3, pages 419–430, June 2014
How to Cite
Kumsta, C., Ching, T.-T., Nishimura, M., Davis, A. E., Gelino, S., Catan, H. H., Yu, X., Chu, C.-C., Ong, B., Panowski, S. H., Baird, N., Bodmer, R., Hsu, A.-L. and Hansen, M. (2014), Integrin-linked kinase modulates longevity and thermotolerance in C. elegans through neuronal control of HSF-1. Aging Cell, 13: 419–430. doi: 10.1111/acel.12189
- Issue published online: 23 MAY 2014
- Article first published online: 9 JAN 2014
- Accepted manuscript online: 6 DEC 2013 07:09AM EST
- Manuscript Accepted: 24 NOV 2013
- Office of Research Infrastructure Programs. Grant Number: P40 OD010440
- Ellison Foundation New Scholar in Aging Award
- Glenn Award for Research in Biological Mechanisms of Aging
- American Association for Cancer Research/Astellas Pharma Global Development Inc
- Basic Cancer Research
- Ellison Medical Foundation/AFAR
- National Institutes of Health (NIA)
Fig. S1 pat-4/ILK mRNA levels are reduced in C. elegans by whole-life and adult-only RNAi treatments.
Fig. S2 Effects of reduction of integrin-complex components in C. elegans expressing GFP-tagged PAT-4/ILK.
Fig. S3 Effects on stress-inducible GFP reporters following inhibition of pat-4/ILK in adult C. elegans transcription factor mutants.
Fig. S4 pat-4/ILK mRNA levels are reduced in hsf-1 mutants.
Fig. S5 Reduction of pat-4/ILK in gcy-8 and ttx-3 mutants does not increase transcription of HSF-1 target genes.
Fig. S6 Thermotactic behavior is intact in C. elegans with reduced PAT-4/ILK levels.
Fig. S7 Whole-life inhibition of pat-4/ILK increases aggregation of polyglutamine-expansion repeats in C. elegans.
Table S1 Lifespan analysis of various strains subjected to pat-4/ILK RNAi.
Table S2 Survival analysis of wild-type animals subjected to RNAi against components of the integrin complex and cytoskeleton during adulthood and incubated at 36°C.
Table S3 Summary of phenotypes observed in animals subjected to RNAi of integrin complex and cytoskeletal components.
Table S4 Survival analysis of transcription factor mutants subjected to pat-4/ILK RNAi during adulthood and incubated at 36°C.
Table S5 Survival analysis of mutants with defects in neurons involved in thermosensation subjected to pat-4/ILK RNAi during adulthood and incubated at 36°C.
Table S6 Survival analysis of gon-2 mutants subjected to pat-4/ILK RNAi during adulthood and incubated at 36°C.
Table S7 C. elegans strains used in this study.
Table S8 Sequences of quantitative RT-PCR primers used in this study.
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