These authors contributed equally.
Altered proteome turnover and remodeling by short-term caloric restriction or rapamycin rejuvenate the aging heart
Article first published online: 25 FEB 2014
© 2014 The Authors. Aging Cell Published by the Anatomical Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 13, Issue 3, pages 529–539, June 2014
How to Cite
Dai, D.-F., Karunadharma, P. P., Chiao, Y. A., Basisty, N., Crispin, D., Hsieh, E. J., Chen, T., Gu, H., Djukovic, D., Raftery, D., Beyer, R. P., MacCoss, M. J. and Rabinovitch, P. S. (2014), Altered proteome turnover and remodeling by short-term caloric restriction or rapamycin rejuvenate the aging heart. Aging Cell, 13: 529–539. doi: 10.1111/acel.12203
- Issue published online: 23 MAY 2014
- Article first published online: 25 FEB 2014
- Manuscript Accepted: 5 JAN 2014
- NIH. Grant Numbers: P30 AG013280, RO1 HL101186, RO1 AG038550
- Ellison Medical Foundation. Grant Number: AG-SS-2535-10
- American Federation for Aging Research Breakthroughs in Gerontology
Fig. S1 Body weights of mice over the course of the experiment.
Fig. S2 Topograph plot for turnover analysis.
Fig. S3 mTOR Western.
Fig. S4 Mitochondrial copy number and mitochondrial biogenesis markers did not increase with CR or RP.
Fig. S5 Precursor pool increased over time in all experimental group.
Fig. S6 Distribution of relative standard error of half-lives.
Fig. S7 Linear regression fits of peptide abundance (peptide isotopologue area) over the labeling period indicate no significant change.
Fig. S8 Topograph LOESS regression of chromatogram alignment for abundance analysis.
|acel12203-sup-0002-SupplementaryMethods.doc||Word document||82K||Method S1 Altered proteome turnover and remodeling by subacute caloric restriction and rapamycin rejuvenate the aging heart|
Table S1 Slope of proteome dynamics (turnover)
Table S2 (A) Pathway analysis of YCL versus OCL. (B) Pathway analysis of OCR versus OCL. (C) Pathway analysis of ORP versus OCL.
Table S3 Protein abundance ratio (log2 of fold change)
Table S4 Top pathway abundance ratio (for heat map)
Table S5 Top pathway half-life ratios (for heat map)
Table S6 Metabolic profiling
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.