Modulated expression of human peripheral blood microRNAs from infancy to adulthood and its role in aging

Authors

  • Chi-Yu Lai,

    1. Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan
    2. Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
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  • Yen-Tzu Wu,

    1. School and Graduate Institute of Physical Therapy, National Taiwan University College of Medicine, Taipei, Taiwan
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  • Sung-Liang Yu,

    1. Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
    2. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan
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  • Ya-Hui Yu,

    1. Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
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  • Su-Yin Lee,

    1. Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
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  • Chih-Min Liu,

    1. Department of Psychiatry, College of Medicine and National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan
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  • Wu-Shiun Hsieh,

    1. Department of Pediatrics, College of Medicine and National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan
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  • Hai-Gwo Hwu,

    1. Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan
    2. Department of Psychiatry, College of Medicine and National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan
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  • Pau-Chung Chen,

    1. Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan
    2. Department of Environmental and Occupational Medicine, College of Medicine and National Taiwan University Hospital, Taipei 100, Taiwan
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  • Suh-Fang Jeng,

    1. School and Graduate Institute of Physical Therapy, National Taiwan University College of Medicine, Taipei, Taiwan
    2. Physical Therapy Center, National Taiwan University Hospital, Taipei, Taiwan
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  • Wei J. Chen

    Corresponding author
    1. Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan
    2. Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
    3. Department of Psychiatry, College of Medicine and National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan
    • Correspondence

      Wei J. Chen, Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, 17 Xu-Zhou Road, Taipei 100, Taiwan. Tel: +886-2-33668010; fax: +886-2-33668004;e-mail: wjchen@ntu.edu.tw

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Summary

Accumulating evidence suggests a role for microRNAs (miRNAs) in regulating various processes of mammalian postnatal development and aging. To investigate the changes in blood-based miRNA expression from preterm infants to adulthood, we compared 365 miRNA expression profiles in a screening set of preterm infants and adults. Approximately one-third of the miRNAs were constantly expressed from postnatal development to adulthood, another one-third were differentially expressed between preterm infants and adults, and the remaining one-third were not detectable in these two groups. Based on their expression in infants and adults, the miRNAs were categorized into five classes, and six of the seven miRNAs chosen from each class except one with age-constant expression were confirmed in a validation set containing infants, children, and adults. Comparing the chromosomal locations of the different miRNA classes revealed two hot spots: the miRNA cluster on 14q32.31 exhibited age-constant expression, and the one on 9q22.21 exhibited up-regulation in adults. Furthermore, six miRNAs detectable in adults were down-regulated in older adults, and four chosen for individual quantification were verified in the validation set. Analysis of the network functions revealed that differentially regulated miRNAs between infants and adults and miRNAs that decreased during aging shared two network functions: inflammatory disease and inflammatory response. Four expression patterns existed in the 11 miRNAs from infancy to adulthood, with a significant transition in ages 9–20 years. Our results provide an overview on the regulation pattern of blood miRNAs throughout life and the possible biological functions performed by different classes of miRNAs.

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