Bmi-1 plays a critical role in protection from renal tubulointerstitial injury by maintaining redox balance
Version of Record online: 11 JUN 2014
© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 13, Issue 5, pages 797–809, October 2014
How to Cite
Jin, J., Lv, X., Chen, L., Zhang, W., Li, J., Wang, Q., Wang, R., Lu, X. and Miao, D. (2014), Bmi-1 plays a critical role in protection from renal tubulointerstitial injury by maintaining redox balance. Aging Cell, 13: 797–809. doi: 10.1111/acel.12236
- Issue online: 19 SEP 2014
- Version of Record online: 11 JUN 2014
- Manuscript Accepted: 28 APR 2014
- National Natural Science Foundation of China. Grant Number: 81200491
- National Basic Research Program of China. Grant Number: 2012CB966902
- Jiangsu Province Ordinary University Innovative Research Project. Grant Number: CXZZ12_0557
- Nanjing Medical University Science and Technology Development Fund Project. Grant Number: 2010NJMU036
Fig. S1 Western blots of kidney extracts for expressions of (A) α-SMA, (C) γ-H2A.X, CHK2, pCHK2, (E) p16, p19, p53 and p21. (B) α-SMA, (D) γ-H2A.X, CHK2, pCHK2, (F) p16, p19, p53 and p21 protein levels relative to β-actin protein levels were assessed by densitometric analysis. β-actin was used as a loading control.
Fig. S2 Enhanced EMT induced by oxidative stress were ameliorated by NAC treatment in Bmi-1-knockdown HK2 cells.
Fig. S3 Renal interstitial fibrosis and tubule atrophy are ameliorated by pyrro-quinoline quinone (PQQ) treatment in Bmi-1-/- mice.?
Fig. S4 Renal tubulointerstitial inflammation and redox imbalance were ameliorated by pyrro-quinoline quinone (PQQ) treatment in Bmi-1-/- mice.
Fig. S5 Renal redox imbalance is not ameliorated by deletion of p16 in Bmi-1-/- mice (Bmi-1-/-p16-/-).
Table S1 siRNAs against human Bmi-1.
Table S2 Primers for RT–PCR and Real-time RT–PCR.
Table S3 Lifetime survival studies.
|acel12236-sup-0003-CompleteExperimentalProcedures.doc||Word document||129K||Appendix S1 Complete experimental procedures and references.|
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