SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
acel12236-sup-0001-FigS1-S5.pdfapplication/PDF6473K

Fig. S1 Western blots of kidney extracts for expressions of (A) α-SMA, (C) γ-H2A.X, CHK2, pCHK2, (E) p16, p19, p53 and p21. (B) α-SMA, (D) γ-H2A.X, CHK2, pCHK2, (F) p16, p19, p53 and p21 protein levels relative to β-actin protein levels were assessed by densitometric analysis. β-actin was used as a loading control.

Fig. S2 Enhanced EMT induced by oxidative stress were ameliorated by NAC treatment in Bmi-1-knockdown HK2 cells.

Fig. S3 Renal interstitial fibrosis and tubule atrophy are ameliorated by pyrro-quinoline quinone (PQQ) treatment in Bmi-1-/- mice.?

Fig. S4 Renal tubulointerstitial inflammation and redox imbalance were ameliorated by pyrro-quinoline quinone (PQQ) treatment in Bmi-1-/- mice.

Fig. S5 Renal redox imbalance is not ameliorated by deletion of p16 in Bmi-1-/- mice (Bmi-1-/-p16-/-).

acel12236-sup-0002-TableS1-S3.docWord document130K

Table S1 siRNAs against human Bmi-1.

Table S2 Primers for RT–PCR and Real-time RT–PCR.

Table S3 Lifetime survival studies.

acel12236-sup-0003-CompleteExperimentalProcedures.docWord document129KAppendix S1 Complete experimental procedures and references.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.