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Fig. S1 The structure of the lung during aging presented by SkyScan micro Computed Tomograph; photographs of lungs of (A): 1–month-old and (B): 24-month-old Balb/c mice were obtained in 180 degree and converted to 3D by CTVol software (Skyscan).

Fig. S2 Characterization of 3D lung micro-tissue model.

Fig. S3 Inflammatory cytokines in 2D vs 3D tissues.

Fig S4 Pro-surfactant protein C expression following rhWnt5a treatment of the 3D human lung tissue model.

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