acel12249-sup-0001-SupplementaryMaterial.docxWord document1332K

Method S1 Plasmid and retroviruses.

Table S1 Oligonucleotides used in this study.

Fig. S1 PIM-1 Expression is Up-regulated in senescent WI38 cells.

Fig. S2 Ectopic Expression of PIM-1 Causes Premature Senescence.

Fig. S3 PIM-1 depletion delays cellular senescence in BJ cells.

Fig. S4 PIM-1 knockdown diminishes the DNA-Damage Response of cells to RasV12.

Fig. S5 Anti-pHP1γS93 antibody reacts in western blot analysis with the WT HP1γ protein but not with the S93A mutant, when exposed to rPIM-1 (top panel).

Fig. S6 PKA activity in young and Ras-induced senescent cells.

Fig. S7 Phosphorylated HP1γ that is associated with cellular senescence.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.