Fibroblasts derived from long-lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress

Authors

  • Melissa M. Page,

    1. Integrative and Environmental Physiology, Institute of Biology and Environmental Sciences, University of Aberdeen, Aberdeen, UK
    Current affiliation:
    1. Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada
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  • Amy Sinclair,

    1. Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medicine, Veterinary and Life Sciences, Graham Kerr Building, University of Glasgow, Glasgow, UK
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  • Ellen L. Robb,

    1. Department of Biological Sciences and Cold Climate Oenology and Viticulture Institute, Brock University, St. Catharines, ON, Canada
    Current affiliation:
    1. MRC Mitochondrial Biology Unit, Mitochondrial Dysfunction, Cambridge, UK
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  • Jeffrey A. Stuart,

    1. Department of Biological Sciences and Cold Climate Oenology and Viticulture Institute, Brock University, St. Catharines, ON, Canada
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  • Dominic J. Withers,

    1. Metabolic Signaling Group, Medical Research Council Clinical Sciences Centre, Imperial College, London, UK
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  • Colin Selman

    Corresponding author
    1. Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medicine, Veterinary and Life Sciences, Graham Kerr Building, University of Glasgow, Glasgow, UK
    • Correspondence

      Colin Selman, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medicine, Veterinary and Life Sciences, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ, UK. Tel.: +44 (0) 141 330 6077; fax: +44 (0) 141 330 5971; e-mail: Colin.Selman@glasgow.ac.uk

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Summary

Reduced signalling through the insulin/insulin-like growth factor-1 signalling (IIS) pathway is a highly conserved lifespan determinant in model organisms. The precise mechanism underlying the effects of the IIS on lifespan and health is currently unclear, although cellular stress resistance may be important. We have previously demonstrated that mice globally lacking insulin receptor substrate 1 (Irs1−/−) are long-lived and enjoy a greater period of their life free from age-related pathology compared with wild-type (WT) controls. In this study, we show that primary dermal fibroblasts and primary myoblasts derived from Irs1−/− mice are no more resistant to a range of oxidant and nonoxidant chemical stressors than cells derived from WT mice.

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