Fibroblasts derived from long-lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress
Article first published online: 24 JUL 2014
© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
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Volume 13, Issue 5, pages 962–964, October 2014
How to Cite
Page, M. M., Sinclair, A., Robb, E. L., Stuart, J. A., Withers, D. J. and Selman, C. (2014), Fibroblasts derived from long-lived insulin receptor substrate 1 null mice are not resistant to multiple forms of stress. Aging Cell, 13: 962–964. doi: 10.1111/acel.12255
- Issue published online: 19 SEP 2014
- Article first published online: 24 JUL 2014
- Manuscript Accepted: 24 JUN 2014
- BBSRC New Investigator. Grant Number: BB/H012850/2
- Wellcome Trust Strategic Award. Grant Number: 098565/Z/12/Z
Table S1 LD50 values of dermal fibroblasts isolated from WT and Irs1−/− mice exposed to lethal oxidative and non-oxidative stressors; cultured at 3% and 21% O2.
Table S2 LD50 values of myoblasts isolated from WT and Irs1−/− mice exposed to lethal oxidative stressors; cultured at 3% O2.
Table S3 Primers used in the present study.
Fig. S1 Fibroblasts from Irs1−/− mice are not more resistant to lethal stress than fibroblasts from WT mice at 3% O2.
Fig. S2 Fibroblasts from Irs1−/− mice do not differ in terms of (A) proliferation rates, (B) cell cycle progression or (C) growth rates after 24 h or 48 h of culture (21% O2) compared with cells derived from WT mice (n = 4 per group).
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