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Original Article

Similar Ethanol Drinking in Adolescent and Adult C57BL/6J Mice After Chronic Ethanol Exposure and Withdrawal

  1. Priscila F. Carrara-Nascimento1,
  2. Marcelo F. Lopez2,
  3. Howard C. Becker2,
  4. M. Foster Olive3,
  5. Rosana Camarini1,*

Article first published online: 8 JAN 2013

DOI: 10.1111/acer.12056

Issue

Cover image for Vol. 37 Issue 5

Additional Information

How to Cite

Carrara-Nascimento, P. F., Lopez, M. F., Becker, H. C., Olive, M. F. and Camarini, R. (2013), Similar Ethanol Drinking in Adolescent and Adult C57BL/6J Mice After Chronic Ethanol Exposure and Withdrawal. Alcoholism: Clinical and Experimental Research. doi: 10.1111/acer.12056

Author Information

  1. 1

    Departamento de Farmacologia , Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil

  2. 2

    Department of Psychiatry and Behavioral Sciences , Medical University of South Carolina, Charleston, South Carolina

  3. 3

    Department of Psychology , Arizona State University, Tempe, Arizona

*Reprint requests: Rosana Camarini, PhD, Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1524, São Paulo, SP 05508-900, Brazil; Tel.:/Fax: +55 11 3091 7756; E-mail: camarini@icb.usp.br

Publication History

  1. Article first published online: 8 JAN 2013
  2. Manuscript Accepted: 11 OCT 2012
  3. Manuscript Received: 15 MAY 2012

Funded by

  • Fundação de Amparo à Pesquisa do Estado de São Paulo. Grant Numbers: AA013852, AA014095
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico

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Keywords:

  • Ethanol Drinking;
  • Chronic Ethanol Exposure;
  • Adolescent;
  • Withdrawal;
  • C57BL/6J mice

Background

Increasing evidence shows that excessive alcohol consumption during adolescence increases vulnerability to alcohol use disorders in adulthood. The aim of this study was to examine differences between adolescent and adult C57BL/6J mice in drinking behavior and blood ethanol (EtOH) concentrations (BECs) after chronic EtOH exposure and withdrawal.

Methods

Male adolescent (PND = 28 to 30) and adult (PND = 70) C57BL/6J mice were allowed to consume EtOH in a 2-bottle choice paradigm (15% EtOH vs. water) for 3 weeks (Baseline drinking, Test 1, and Test 2), which were interspersed with 2 cycles (Cycles I and II) of chronic EtOH vapor or air inhalation (16 hours) and withdrawal (8 hours). BECs were determined during both cycles.

Results

Chronic EtOH exposure led to increased EtOH intake during Test 1 and Test 2 in both adolescent and adult mice compared with air-exposed controls, and no differences between age groups were observed. During Cycle I adult mice showed higher BECs compared with adolescents. During Cycle II, BECs were lower in adult mice as compared to Cycle I, and BECs in adolescent mice did not change between the 2 cycles.

Conclusions

Chronic EtOH exposure followed by withdrawal periods increases EtOH consumption similarly in both adolescent and adult mice, despite differences in BECs.

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