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Adipose Inflammation and Macrophage Infiltration After Binge Ethanol and Burn Injury

Authors

  • Yuanyuan Qin,

    1. Nutrition Obesity Research Center, Departments of Nutrition and Medicine, Gillings School of Global Public Health and School of Medicine, UNC Chapel Hill, Chapel Hill, North Carolina
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  • Jillian L. Hamilton,

    1. Nutrition Obesity Research Center, Departments of Nutrition and Medicine, Gillings School of Global Public Health and School of Medicine, UNC Chapel Hill, Chapel Hill, North Carolina
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  • Melanie D. Bird,

    1. Alcohol Research Program, Department of Surgery, Burn & Shock Trauma Research Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois
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  • Michael M. Chen,

    1. Alcohol Research Program, Department of Surgery, Burn & Shock Trauma Research Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois
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  • Luis Ramirez,

    1. Alcohol Research Program, Department of Surgery, Burn & Shock Trauma Research Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois
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  • Anita Zahs,

    1. Alcohol Research Program, Department of Surgery, Burn & Shock Trauma Research Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois
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  • Elizabeth J. Kovacs,

    1. Alcohol Research Program, Department of Surgery, Burn & Shock Trauma Research Institute, Loyola University Chicago, Stritch School of Medicine, Maywood, Illinois
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  • Liza Makowski

    Corresponding author
    1. Nutrition Obesity Research Center, Departments of Nutrition and Medicine, Gillings School of Global Public Health and School of Medicine, UNC Chapel Hill, Chapel Hill, North Carolina
    • Reprint requests: Liza Makowski, PhD, CB 7461 Department of Nutrition, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2203 McGavran Greenberg Hall, Chapel Hill, NC 27599-7461; Tel.: 919-843-4383; Fax: 919-966-7216; E-mail: liza.makowski@unc.edu

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Abstract

Background

Ethanol (EtOH) exposure prior to traumatic injury, such as a burn, elevates systemic and local inflammatory responses and increases morbidity and mortality. Adipose is a large tissue mass that is often inflamed during obesity or other stresses, which disturbs metabolic homeostasis. To date, there has been little investigation into the inflammatory response of adipose tissue after combined EtOH exposure and burn injury.

Methods

Two EtOH exposure regimens were utilized to examine the role of inflammation in adipose tissue after EtOH and burn injury. Mice were either given a single or episodic binge exposure to EtOH or saline followed by scald (burn) or sham injury 30 minutes later. Twenty-four hours post injury, serum and adipose tissue were collected for assessment of inflammatory mediators.

Results

Single binge EtOH alone induced no inflammation in adipose when compared with sham vehicle-treated mice. However, single binge EtOH followed by burn injury induced significant elevations in mRNA and protein concentrations of pro-inflammatory mediators interleukin-6 (IL-6), KC, and monocyte chemoattractant protein 1 compared with either insult alone or sham vehicle group. Additionally, EtOH exposure and burn injury significantly blunted inducible nitric oxide synthase (iNOS), indicating a complex inflammatory response. Episodic binge EtOH exposure followed by burn injury exacerbated the postburn adipose inflammatory response. The magnitude of the episodic binge-induced inflammatory parameters postburn were 2- to 5-fold greater than the response detected after a single exposure of EtOH, indicating EtOH-induced potentiation of burn-induced inflammatory response. Finally, inflammatory loci and crown-like structures in adipose were significantly increased by episodic binge EtOH and burn injury.

Conclusions

This is the first report of binge and burn-induced crown-like structure formation. Evidence presented herein suggests an important role for alcohol and burn as an additional mediator of adipose inflammation in postburn injury, a common complication in burn patients.

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