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Table S1. PCR and SBE primers used for the 15-plex iPLEX assay (Sequenom).

Table S2. References of the ancient and contemporary Amerindian populations included in the mtDNA analyses.

Table S3. References of the modern day South American populations used for comparison and analyses of Y chromosome data.

Table S4. FST values, from mtDNA and Y-chromosome data, calculated between Humahuaca and ancient and modern South American populations.

Figure S1. Median Joining Networks analyses on mitochondrial haplogroups A2, B2, C1, and D1.

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