Background and Aims
New high-throughput tools for transcriptomic analysis such as RNA sequencing have been developed rapidly in recent years. These technologies provide new opportunities for biologists to improve the understanding of gene expression underlying important physiological processes. The sequencing of RNA by next-generation technologies is, in particular, dependent on the use of pure and un-degraded RNA. Many fruits from perennial crops share an elevated concentration of tannins and polysaccharides and a strong dilution of cytosolic content upon vacuole hypertrophy. The grapevine berry presents these characteristics together with high vacuole acidity, which has never been taken into account in previous protocols, and poses thereby a particular challenge for RNA extraction.