Analysis of HLA-G Polymorphisms in Couples with Implantation Failure
Article first published online: 25 SEP 2012
© 2012 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 68, Issue 6, pages 507–514, December 2012
How to Cite
Analysis of HLA-G polymorphisms in couples with implantation failure. Am J Reprod Immunol 2012; 00: 00–00, , , , , , , .
- Issue published online: 8 NOV 2012
- Article first published online: 25 SEP 2012
- Manuscript Accepted: 9 JUL 2012
- Manuscript Received: 16 APR 2012
- Assisted reproduction treatment;
- HLA-G; soluble HLA-G;
- implantation failure
HLA-G expression is related as an immune modulator of fetal–maternal tolerance, and its levels was correlated with pregnancy outcome. In a case–control study, we investigate the association between the genetic variability of the HLA-G gene and serum levels of soluble HLA-G in cases of embryo implantation failure.
Method of Study
Forty couples with at least two unsuccessful fresh embryo transfers (implantation failure; IF) and 83 fertile couples with at least two successful pregnancies was genotyped by sequencing-based typing. HLA-G alleles were defined by nucleotide sequence variations at exon 2, 3, and 4, and the quantification of soluble HLA-G (sHLA-G) was performed by ELISA.
There was a significant difference between the HLA-G allelic distributions between IF couples and the control couples. The HLA-G*01:03:01 allele was increased in the IF couples. There were no significant differences in the serum levels of sHLA-G in the IF and control groups.
The results suggest that the distribution of HLA-G products may play a significant role in the modulation of maternal–fetal immune response.