Trophoblastic Cell Lines ACH1P and AC-1M32 React with a Distinctive Cytokine Pattern Toward Listeria Monocytogenes and Show Morphologic Differences
Article first published online: 14 AUG 2012
© 2012 John Wiley & Sons A/S
American Journal of Reproductive Immunology
Volume 68, Issue 5, pages 387–391, November 2012
How to Cite
Trophoblastic cell lines ACH1P and AC-1M32 react with a distinctive cytokine pattern toward listeria monocytogenes and show morphologic differences. Am J Reprod Immunol 2012; 68: 387–391, , , .
- Issue published online: 10 OCT 2012
- Article first published online: 14 AUG 2012
- Manuscript Accepted: 10 JUL 2012
- Manuscript Received: 22 MAR 2012
- Listeria monocytogenes;
The differential reaction of cytotrophoblast and syncytiotrophoblast towards infection with listeria monocytogenes (LM) is pivotal to its pathogenicity. In this study we tested the cytokine signature upon infection with listeria monocytogenes (LM) in an in vitro model.
Method of study
We compared two related trophoblastic cell lines (AC-1M32 and ACH1P). The cell line ACH1P showed syncytium formation, whereas AC-1M32 did not fuse, as demonstrated with immunfluorescence E-Cadherin staining. In a Multi-Analyte ELISArray we tested for concentrations of TNFα, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, IL-17A, IL-8, MCP1, MIP-1a, MIP-1b, MDC, Eotaxin, IFNγ, G-CSF, TGFβ1 after 8 and 24 hours.
Compared to unstimulated cells, the syncytial cell line ACH1P showed a significant induction of Interleukin-6 (IL-6), Monocyte Chemotactic Protein-1 (MCP-1) and transforming growth factor β-1 (TGFβ1). Incubating AC-1M32 with LM, however, showed significantly reduced IL-6 levels and a massively increased (~300fold) TGFβ1 secretion compared to unstimulated controls.
A functional anti-LM immune response was only induced by the syncytium-forming cell line ACH1P. Using the two sister cell lines AC-1M32 and ACH1P in an in vitro LM-stimulation assay might facilitate research in the area of placental listeria infection.