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Keywords:

  • Peroxiredoxin 3;
  • peroxiredoxin 4;
  • placenta;
  • proteomics;
  • recurrent pregnancy loss

Problem

Recurrent pregnancy loss (RPL) is defined as two or more consecutive abortion before the 20th week of gestation. Autoimmune diseases increase the risk and accounts for at least 20% of RPL. Placenta is a pregnancy unique tissue, and proper formation of placenta is key phenomenal for success of a pregnancy. The aim of this study was to investigate the placental proteins that may act as antibody targets in RPL patients.

Method of study

Total placental proteins were extracted and separated using 2D-PAGE technique. Separated protein spots were transferred on PVDF membrane and blotted with sera from 20 RPL patients and compared with the protein spots that membrane blotted with sera from 20 normal women. Differentially blotted spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry mass spectrometry technique. The results of the mass analysis also were confirmed by western blot using mAb and reverse transcriptase polymerase chain reaction technique.

Results

The results indicated that RPL women may produce antibody against Peroxiredoxin 3 (Prx3) and Peroxiredoxin 4 (Prx4).

Conclusion

Our results indicate that two placental proteins, Prx3 and Prx4, may act as new placental immune targets. Considering the role of antioxidant defense in the protection of placenta from oxidative stress, production of antibodies against peroxiredoxins 3 and 4 may introduce a new autoimmune hypothesis in RPL, which is needed to be tested in the future works.